Diagnostic Accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for Diagnosis of Leishmaniasis in Sudan 英文参考文献.docVIP

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Diagnostic Accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for Diagnosis of Leishmaniasis in Sudan 英文参考文献.doc

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Diagnostic Accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for Diagnosis of Leishmaniasis in Sudan 英文参考文献

DiagnosticAccuracyoftheLeishmaniaOligoC-TesTand NASBA-OligochromatographyforDiagnosisof LeishmaniasisinSudan AlfarazdegA.Saad1,NuhaG.Ahmed1,OsmanS.Osman1,AhmedAlmustafaAl-Basheer2,AwadHamad2, StijnDeborggraeve3,4*,PhilippeBu¨scher3,GerardJ.Schoone5,HenkD.Schallig5,ThierryLaurent6, AhmedHaleem7,OmranF.Osman8,AhmedMohamedainEltom1,MustafaI.Elbashir1,SaydaEl-Safi2 1DepartmentofBiochemistry,KhartoumUniversity,Sudan,2DepartmentofMicrobiologyandParasitology,KhartoumUniversity,Sudan,3DepartmentofParasitology, InstituteofTropicalMedicine,Antwerp,Belgium,4RegaInstitute,KatholiekeUniversiteitLeuven,Leuven,Belgium,5KITBiomedicalResearch,ParasitologyUnit,Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands, 6Coris BioConcept, Gembloux, Belgium, 7Faculty of Laboratory Science, Khartoum University, Sudan, 8FacultyofScience,KhartoumUniversity,Sudan Abstract Background: The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified andstandardisedmoleculardetectionofLeishmaniaparasitesinclinicalspecimens.WeherepresentthephaseIIevaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leishmaniasis (CL) and post kala-azar dermal leishmaniasis(PKDL)inSudan. Methodology:Thediagnosticaccuracyofthetestswasevaluatedon90confirmedand90suspectedVLcases,7confirmed and8suspectedCLcases,2confirmedPKDLcasesand50healthyendemiccontrolsfromGedarifstateandKhartoumstate inSudan. Principal Findings: The OligoC-TesT as well as the NASBA-OC showed a sensitivity of 96.8% (95% CI: 83.8%–99.4%) on lymphnodeaspiratesandof96.2%(95%CI:89.4%–98.7%)onbloodfromtheconfirmedVLcases.Thesensitivityonbone marrow was 96.9% (95% CI: 89.3%–99.1%) and 95.3% (95% CI: 87.1%–98.4%) for the OligoC-TesT and NASBA-OC, respectively. All confirmed CL andPKDLcases were positivewith both tests. Onthesuspected VLcases, we observeda positiveOligoC-TesTandNASBA-OCresultin37.1%(95%CI:23.2%–53.7%)and34.3%(95%CI:20.8%–50.9%)onlymph,in 72.7%(95%CI:55.8%–84.9