Differences between CusA and AcrB Crystallisation Highlighted by Protein Flexibility 英文参考文献.docVIP

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Differences between CusA and AcrB Crystallisation Highlighted by Protein Flexibility 英文参考文献.doc

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Differences between CusA and AcrB Crystallisation Highlighted by Protein Flexibility 英文参考文献

DifferencesbetweenCusAandAcrBCrystallisation HighlightedbyProteinFlexibility Aure′lienDeniaud,Aure′lieGoulielmakis,JacquesCove`s,EvaPebay-Peyroula* InstitutdeBiologieStructuraleJeanPierreEbel,UMR5075CEA-CNRS-Universite′ JosephFourier,Grenoble,France Abstract Background: Until very recently, AcrB was the only Resistance Nodulation and cell Division transporter for which the structurehasbeenelucidated.Towardsageneralunderstandingofthisproteinfamily,CusAandAcrBwerecompared. Methodology/PrincipalFindings:Indodecylmaltoside,AcrBcrystallisedinmanydifferentconditions,whileCusAdoesnot. Thiscouldbeduetothedifferenceindynamicbetweentheseproteinsasjudgedfromlimitedproteolysisassays.Addition ofvariouscompounds,inparticularheavymetalcations,stabilisesCusA. Conclusion/Significance:ThisapproachcouldconstituteafirststeptowardsCusAcrystallisation. Citation:DeniaudA,GoulielmakisA,Cove`sJ,Pebay-PeyroulaE(2009)DifferencesbetweenCusAandAcrBCrystallisationHighlightedbyProteinFlexibility.PLoS ONE4(7):e6214.doi:10.1371/journal.pone.0006214 Editor:AndreasHofmann,GriffithUniversity,Australia ReceivedMarch20,2009;AcceptedJune8,2009;PublishedJuly10,2009 Copyright: ? 2009 Deniaud et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by the Commissariat a` l’E′nergie Atomique, the Centre National de la Recherche Scientifique, Universite′ Joseph-Fourier Grenoble and E.U. Specific Targeted Research Project IMPS (Innovative tools for membrane protein structural proteomics). The funders had no role in study design,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:eva.pebay-peyroula@ibs.fr Introduction copper resistance are located in the second large periplasmic domain[5]. As any gram-neg