Different Capacity of Monocyte Subsets to Phagocytose Iron-Oxide Nanoparticles 英文参考文献.docVIP

下载本文档

2
0
约4.65万字
约 9页
2017-05-11 发布于上海
举报
版权申诉

Different Capacity of Monocyte Subsets to Phagocytose Iron-Oxide Nanoparticles 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Different Capacity of Monocyte Subsets to Phagocytose Iron-Oxide Nanoparticles 英文参考文献

DifferentCapacityofMonocyteSubsetstoPhagocytose Iron-OxideNanoparticles MarcusSettles1,MartinEtzrodt2,KatjaKosanke1,MatthiasSchiemann3,AlexanderZimmermann4, ReinhardMeier1,RickmerBraren1,ArminHuber1,ErnstJ.Rummeny1,RalphWeissleder2,FilipK. Swirski2,MoritzWildgruber1,2* 1Institutfu¨rRadiologie,KlinikumRechtsderIsar,TechnischeUniversita¨t Mu¨nchen,Munich,Germany,2CenterforSystemsBiology,MassachusettsGeneralHospitaland HarvardMedicalSchool,Boston,Massachusetts,UnitedStatesofAmerica,3Institutfu¨rmedizinischeMikrobiologie,ImmunologieundHygiene,KlinikumRechtsderIsar, Technische Universita¨t Mu¨nchen and Clinical Cooperation Group ‘‘Antigen-Specific Immunotherapy’’, Helmholtzzentrum Mu¨nchen, Munich, Germany, 4Klinik und Poliklinikfu¨rGefa¨?chirurgie, KlinikumRechtsderIsar,TechnischeUniversita¨t Mu¨nchen,Munich,Germany Abstract Objective: To explore the capacity of human CD14+CD16++ and CD14++CD16- monocytes to phagocyte iron-oxide nanoparticlesinvitro. Methods:Humanmonocyteswerelabeledwithfourdifferentmagneticnanoparticlepreparations(Ferumoxides,SHU555C, CLIO-680, MION-48) exhibiting distinct properties and cellular uptake was quantitatively assessed by flow cytometry, fluorescence microscopy, atomic absorption spectrometry and Magnetic Resonance Imaging (MRI). Additionally we determinedwhethercellularuptakeofthenanoparticlesresultedinphenotypicchangesofcellsurfacemarkers. Results: Cellular uptake differed between the four nanoparticle preparations. However for each nanoparticle tested, ++ + ++ CD14 CD16- monocytes displayed a significantly higher uptake compared to CD14 CD16 monocytes, this resulted in significantly lower T1 and T2 relaxation times of these cells. The uptake of iron-oxide nanoparticles further resulted in a remarkable shift of expression of cell surface proteins indicating that the labeling procedure affects the phenotype of + CD14 CD16 andCD14 CD16-monocytesdifferently. ++ ++ Conclusion: Human monocyte subsets internalize different magnetic n