Differential Affinity and Catalytic Activity of CheZ in E. coli Chemotaxis 英文参考文献.docVIP

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Differential Affinity and Catalytic Activity of CheZ in E. coli Chemotaxis 英文参考文献.doc

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Differential Affinity and Catalytic Activity of CheZ in E. coli Chemotaxis 英文参考文献

DifferentialAffinityandCatalyticActivityofCheZinE. coliChemotaxis SiebeB.vanAlbada*,PieterReintenWolde FOMInstituteforAtomicandMolecularPhysics,Amsterdam,TheNetherlands Abstract Push–pullnetworks,inwhichtwoantagonisticenzymescontroltheactivityofamessengerprotein,areubiquitousinsignal transductionpathways.AclassicalexampleisthechemotaxissystemofthebacteriumEscherichiacoli,inwhichthekinaseCheA andthephosphataseCheZregulatethephosphorylationlevelofthemessengerproteinCheY.Recentexperimentssuggestthat boththekinaseandthephosphatasearelocalizedatthereceptorcluster,andVakninandBergrecentlydemonstratedthatthe spatialdistributionofthephosphatasecanmarkedlyaffectthedose–responsecurves.Weargue,usingmathematicalmodeling, thatthecanonicalmodelofthechemotaxisnetworkcannotexplaintheexperimentalobservationsofVakninandBerg.We presentanewmodel,inwhichasmallfractionofthephosphataseislocalizedatthereceptorcluster,whiletheremainderfreely diffusesinthecytoplasm;moreover,thephosphataseattheclusterhasahigherbindingaffinityforthemessengerproteinand ahighercatalyticactivitythanthephosphataseinthecytoplasm.Thismodelisconsistentwithalargebodyofexperimental dataandcanexplainmanyoftheexperimentalobservationsofVakninandBerg.Moregenerally,thecombinationofdifferential affinity and catalytic activity provides a generic mechanism for amplifying signals that could be exploited in other two- componentsignalingsystems.Ifthismodeliscorrect,thenanumberofrecentmodelingstudies,whichaimtoexplainthe chemotacticgainintermsoftheactivityofthereceptorcluster,shouldbereconsidered. Citation: van Albada SB, ten Wolde PR (2009) Differential Affinity and Catalytic Activity of CheZ in E. coli Chemotaxis. PLoS Comput Biol 5(5): e1000378. doi:10.1371/journal.pcbi.1000378 Editor:ChristopherRao,UniversityofIllinoisatUrbana-Champaign,UnitedStatesofAmerica ReceivedApril28,2008;AcceptedApril1,2009;PublishedMay8,2009 Copyright: ? 2009 van Albada, ten Wolde. This is an open-access article distributed under the terms of the Cr