Differential Calcium Signaling by Cone Specific Guanylate Cyclase-Activating Proteins from the Zebrafish Retina 英文参考文献.docVIP

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Differential Calcium Signaling by Cone Specific Guanylate Cyclase-Activating Proteins from the Zebrafish Retina 英文参考文献.doc

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Differential Calcium Signaling by Cone Specific Guanylate Cyclase-Activating Proteins from the Zebrafish Retina 英文参考文献

DifferentialCalciumSignalingbyConeSpecific GuanylateCyclase-ActivatingProteinsfromthe ZebrafishRetina AlexanderScholten1,Karl-WilhelmKoch1,2,3* 1Institute of Biology and Environmental Science, Carl von Ossietzky University Oldenburg, Oldenburg, Germany, 2Research Center Neurosensory Science, Carl von OssietzkyUniversityOldenburg,Oldenburg,Germany,3CenterofInterfaceScience,CarlvonOssietzkyUniversityOldenburg,Oldenburg,Germany Abstract Zebrafish express in their retina a higher number of guanylate cyclase-activating proteins (zGCAPs) than mammalians pointingtomorecomplexguanylatecyclasesignalingsystems.AllsixzGCAPisoformsshowdistinctandpartialoverlapping expression profiles in rods and cones. We determined critical Ca -dependent parameters of their functional properties 2+ usingpurifiedzGCAPsafterheterologousexpressioninE.coli.Isoforms1–4werestrong,5and7wereweakactivatorsof membrane bound guanylate cyclase. They further displayed different Ca -sensitivities of guanylate cyclase activation, 2+ 2+ whichishalfmaximaleitheratafreeCa around30nM(zGCAP1,2and3)oraround400nM(zGCAP4,5and7).Zebrafish GCAPisoformsshowedalsodifferencesintheirCa /Mg -dependentconformationalchangesandintheCa -dependent monomer-dimer equilibrium. Direct Ca -binding revealed that allzGCAPs bound at least three Ca . Thecorresponding 2+ 2+ 2+ 2+ 2+ apparentaffinityconstantsreflectbindingofCa2+withhigh(#100nM),medium(0.1–5mM)and/orlow($5mM)affinity, butwereuniqueforeachzGCAPisoform.OurdataindicateaCa -sensorsysteminzebrafishrodandconecellssupporting aCa -relaymodelofdifferentialzGCAPoperationinthesecells. 2+ 2+ Citation: Scholten A,Koch K-W (2011) Differential Calcium Signalingby Cone Specific Guanylate Cyclase-Activating Proteins from the Zebrafish Retina. PLoS ONE6(8):e23117.doi:10.1371/journal.pone.0023117 Editor:StevenBarnes,DalhousieUniversityCanada ReceivedMay21,2011;AcceptedJuly10,2011;PublishedAugust2,2011 Copyright:?2011ScholtenandKoch.Thisisanopen-accessarticledistributedunde