Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans 英文参考文献.docVIP

下载本文档

5
0
约6.82万字
约 14页
2017-05-11 发布于上海
举报
版权申诉

Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans 英文参考文献.doc

1、本文档共14页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans 英文参考文献

DifferentialImpactoftheHEN1HomologHENN-1on21U and26GRNAsintheGermlineofCaenorhabditiselegans LeonieM.Kamminga1.¤a,JosienC.vanWolfswinkel1.¤b,MaartjeJ.Luteijn1.,LucasJ.T.Kaaij1, MarloesP.Bagijn2,AlexandraSapetschnig2,EricA.Miska2,EugeneBerezikov1,Rene′ F.Ketting1* 1Hubrecht Institute–KNAW and University Medical Centre Utrecht, Utrecht, The Netherlands, 2Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge,Cambridge,UnitedKingdom Abstract RNAinterference(RNAi)–relatedpathwaysaffectgeneactivitybysequence-specificrecruitmentofAgoproteinstomRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein.StabilityofsRNAmoleculesinsomepathwaysisinpartregulatedbyHen1-mediatedmethylationoftheir39ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA–methyl-transferase homolog, HENN-1, on the differentRNAipathwaysinthisnematode.WerevealdifferentialeffectsofHENN-1onthetwopathwaysthatareknownto employmethylatedsRNAmolecules:the26Gand21Upathways.Surprisingly,inthegermline,stabilityof21URNAs,theC. eleganspiRNAs,isonlymildlyaffectedbylossofmethylation;andintroductionofartificial21UtargetRNAdoesnotfurther destabilizenon-methylated21URNAs.Incontrast,most26GRNAsdisplayreducedstabilityandrespondtolossofHENN-1 bydisplayingincreased39-uridylationfrequencies.Withinthe26GRNAclass,wefindthatspecificallyERGO-1–bound26G RNAs are modified by HENN-1, while ALG-3/ALG-4–bound 26G RNAs are not. Global gene expression analysis of henn-1 mutantsrevealsmildeffects,includingdown-regulationofmanygermline-expressedgenes.Ourdatasuggestthat,apart fromdirecteffectsofreduced26GRNAlevelsofhenn-1ongeneexpression,mosteffectsonglobalgeneexpressionare indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymesinthesepathways. Citation:KammingaLM,vanWolfswinkelJC,LuteijnMJ,KaaijLJT,BagijnMP,etal.(2012)DifferentialImpactoftheHEN1HomologH