Differential Growth Factor Adsorption to Calvarial Osteoblast-Secreted Extracellular Matrices Instructs Osteoblastic Behavior 英文参考文献.docVIP

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Differential Growth Factor Adsorption to Calvarial Osteoblast-Secreted Extracellular Matrices Instructs Osteoblastic Behavior 英文参考文献.doc

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Differential Growth Factor Adsorption to Calvarial Osteoblast-Secreted Extracellular Matrices Instructs Osteoblastic Behavior 英文参考文献

DifferentialGrowthFactorAdsorptiontoCalvarial Osteoblast-SecretedExtracellularMatricesInstructs OsteoblasticBehavior ArchanaBhat1,SimeonA.Boyadjiev2,CraigW.Senders3,J.KentLeach1* 1Department of Biomedical Engineering, University of California Davis, Davis, California, United States of America, 2Section of Genetics, Department of Pediatrics, UniversityofCaliforniaDavisSchoolofMedicine,Sacramento,California,UnitedStatesofAmerica,3DepartmentofOtolaryngology-HeadandNeckSurgery,Universityof CaliforniaDavisSchoolofMedicine,Sacramento,California,UnitedStatesofAmerica Abstract Craniosynostosis (CS), the premature ossification of cranial sutures, is attributed to increased osteogenic potential of resident osteoblasts, yet the contribution of the surrounding extracellular matrix (ECM) on osteogenic differentiation is unclear.Theosteoblast-secretedECMprovidesbindingsitesforcellularadhesionandregulatesthetransportandsignaling ofosteoinductivefactorssecretedbytheunderlyingduramater.Thebindingaffinityofeachosteoinductivefactorforthe ECMmayamplifyormuteitsrelativeeffect,thuscontributingtotherateofsuturefusion.Thepurposeofthispaperwasto examinetheroleofECMcompositionderivedfromcalvarialosteoblastsonproteinbindinganditsresultanteffectoncell phenotype.Wehypothesizedthatpotentosteoinductiveproteinspresentduringsuturalfusion(e.g.,bonemorphogenetic protein-2 (BMP-2) and transforming growth factor beta-1 (TGF-b1)) would exhibit distinct differences in binding when exposed to ECMs generated by human calvarial osteoblasts from unaffected control individuals (CI) or CS patients. DecellularizedECMsproducedbyosteoblastsfromCIorCSpatientswereincubatedinthepresenceofBMP-2orTGF-b1, andtheaffinityofeachproteinwasanalyzed.ThecontributionofECMcompositiontoproteinbindingwasinterrogatedby enzymaticallymodulatingproteoglycancontentwithintheECM.BMP-2hadasimilarbindingaffinityforeachECM,while TGF-b1hadagreateraffinityforECMsproducedbyosteoblastsfromCIcomparedtoCSpatients.Enzymatictreatmentof ECMs redu