Discovery of Novel dsRNA Viral Sequences by In Silico Cloning and Implications for Viral Diversity, Host Range and Evolution 英文参考文献.docVIP

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Discovery of Novel dsRNA Viral Sequences by In Silico Cloning and Implications for Viral Diversity, Host Range and Evolution 英文参考文献.doc

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Discovery of Novel dsRNA Viral Sequences by In Silico Cloning and Implications for Viral Diversity, Host Range and Evolution 英文参考文献

DiscoveryofNoveldsRNAViralSequencesbyInSilico CloningandImplicationsforViralDiversity,HostRange andEvolution HuiquanLiu1,2¤,YanpingFu2,JiataoXie2,JiasenCheng2,SaidA.Ghabrial3,GuoqingLi1,2,XianhongYi2, DaohongJiang1,2 * 1StateKeyLaboratoryofAgriculturalMicrobiology,HuazhongAgriculturalUniversity,Wuhan,HubeiProvince,People’sRepublicofChina,2TheProvincialKeyLabof Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, People’s Republic of China, 3DepartmentofPlantPathology,UniversityofKentucky,Lexington,Kentucky,UnitedStatesofAmerica Abstract Genomesequenceofvirusescancontributegreatlytothestudyofviralevolution,diversityandtheinteractionbetween virusesandhosts.TraditionalmolecularcloningmethodsforobtainingRNAviralgenomesaretime-consumingandoften difficult because many viruses occur in extremely low titers. DsRNA viruses in the families, Partitiviridae, Totiviridae, Endornaviridae,Chrysoviridae, and other related unclassified dsRNAviruses aregenerally associated with symptomless or persistent infections of their hosts. These characteristics indicate that samples or materials derived from eukaryotic organismsusedtoconstructcDNAlibrariesandESTsequencingmightcarrytheseviruses,whichwerenoteasilydetected by the researchers. Therefore, the EST databases may include numerous unknown viral sequences. In this study, we performedinsilicocloning,aprocedureforobtainingfullorpartialcDNAsequenceofagenebybioinformaticsanalysis, usingknowndsRNAviralsequencesasqueriestosearchagainstNCBIExpressedSequenceTag(EST)database.Fromthis analysis, we obtained 119 novel virus-like sequences related to members of the families, Endornaviridae, Chrysoviridae, Partitiviridae,andTotiviridae.ManyofthemwereidentifiedincDNAlibrariesofeukaryoticlineages,whichwerenotknown to be hosts for these viruses. Furthermore, comprehensive phylogenetic analysis of these newly discovered virus-like sequences with known dsRNA viruses reveale