Diversity and Physiological Characterization of D-Xylose-Fermenting Yeasts Isolated from the Brazilian Amazonian Forest 英文参考文献.docVIP

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Diversity and Physiological Characterization of D-Xylose-Fermenting Yeasts Isolated from the Brazilian Amazonian Forest 英文参考文献.doc

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Diversity and Physiological Characterization of D-Xylose-Fermenting Yeasts Isolated from the Brazilian Amazonian Forest 英文参考文献

DiversityandPhysiologicalCharacterizationofD-Xylose- FermentingYeastsIsolatedfromtheBrazilian AmazonianForest RaquelM.Cadete1,MonalizaA.Melo1,KellyJ.Dussa′n2,RitaC.L.B.Rodrigues2,SilvioS.Silva2, JerriE.Zilli3,MarcosJ.S.Vital4,Fa′timaC.O.Gomes5,Marc-Andre′ Lachance6,CarlosA.Rosa1* 1DepartamentodeMicrobiologia,InstitutodeCie?nciasBiolo′gicas,UniversidadeFederaldeMinasGerais,BeloHorizonte,Brazil,2DepartamentodeBiotecnologia,Escola de Engenharia de Lorena, Universidade de Sa?o Paulo, Sa?o Paulo, Brazil, 3Embrapa Agrobiologia, Serope′dica, Rio de Janeiro, Brazil, 4Departamento de Biologia, UniversidadeFederaldeRoraima,CampusdoParicarana,BoaVista,Brazil,5DepartamentodeQu?′mica,CentroFederaldeEducac?a?o Tecnolo′gicadeMinasGerais,Belo Horizonte,Brazil,6DepartmentofBiology,UniversityofWesternOntario,London,Ontario,Canada Abstract Background:ThisstudyisthefirsttoinvestigatetheBrazilianAmazonianForesttoidentifynewD-xylose-fermentingyeasts thatmightpotentiallybeusedintheproductionofethanolfromsugarcanebagassehemicellulosichydrolysates. Methodology/Principal Findings:A totalof 224yeaststrains wereisolated fromrottingwood samplescollectedin two Amazonian forest reserve sites. These samples were cultured in yeast nitrogen base (YNB)-D-xylose or YNB-xylan media. Candidatropicalis,Asterotremellahumicola,CandidaboidiniiandDebaryomyceshanseniiwerethemostfrequentlyisolated yeasts. Among D-xylose-fermenting yeasts, six strains of Spathaspora passalidarum, two of Scheffersomyces stipitis , and representativesoffivenewspecieswereidentified.ThenewspeciesincludedCandidaamazonensisoftheScheffersomyces clade and Spathaspora sp. 1, Spathaspora sp. 2, Spathaspora sp. 3, and Candida sp. 1 of the Spathaspora clade. In fermentation assays using D-xylose (50g/L) culture medium, S. passalidarum strains showed the highest ethanol yields (0.31g/gto0.37g/g)andproductivities(0.62g/L?hto0.75g/L?h).CandidaamazonensisexhibitedavirtuallycompleteD- xylose consumption and the highest xylito