Diversity of Acinetobacter baumannii in Four French Military Hospitals, as Assessed by Multiple Locus Variable Number of Tandem Repeats Analysis 英文参考文献.docVIP

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Diversity of Acinetobacter baumannii in Four French Military Hospitals, as Assessed by Multiple Locus Variable Number of Tandem Repeats Analysis 英文参考文献.doc

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Diversity of Acinetobacter baumannii in Four French Military Hospitals, as Assessed by Multiple Locus Variable Number of Tandem Repeats Analysis 英文参考文献

DiversityofAcinetobacterbaumanniiinFourFrench MilitaryHospitals,asAssessedbyMultipleLocus VariableNumberofTandemRepeatsAnalysis YolandeHauck1,2,CharlesSoler3,PatrickJault3,AudreyMe′rens4,PatrickGe′rome5,ChristineMacNab3, Franc?oisTrueba6,LaurentBargues3,HoangVuThien7,GillesVergnaud1,2,8,ChristinePourcel1,2 * 1UnivParis-Sud,InstitutdeGe′ne′tiqueetMicrobiologie,UMR8621,Orsay,France,2CNRS,Orsay,France,3Laboratoiredebiologieclinique,HIAPercy,Clamart,France, 4Laboratoiredebiologieclinique,HIABe′gin,SaintMande′,France,5Servicedebiologieme′dicale,HIADesgenettes,Lyon,France,6Servicedebiologieme′dicale,HIAVal deGra?ce,Paris,France,7Bacte′riologie,Ho?pitalArmandTrousseau,AssistancePublique-Ho?pitauxdeParis(APHP),Paris,France,8DGA/MRIS-MissionpourlaRechercheet l’InnovationScientifique,Bagneux,France Abstract Background:InfectionsbyA.calcoaceticus-A.baumannii(ACB)complexisolatesrepresentaseriousthreatforwoundedand burnpatients.Threeinternationalmultidrug-resistant(MDR)clones(EUcloneI-III)areresponsibleforalargeproportionof nosocomialinfectionswithA.baumanniibutotheremergingstrainswithhighepidemicpotentialalsooccur. Methodology/Principal Findings: We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA)protocolandusedittoinvestigatethegeneticdiversityof136ACBisolatesfromfourmilitaryhospitalsandone childrenshospital.Acinetobacterspotherthanbaumanniiisolatesrepresented22.6%(31/137)withamajoritybeingA.pittii. The genotyping protocol designed for A.baumannii was also efficient to cluster A. pittii isolates. Fifty-five percent of A. baumanniiisolatesbelongedtothetwointernationalclonesIandII,andweidentifiednewcloneswhichmemberswere found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogeneticinformationtohelptracebacktheiremergence. Conclusions/Significance:TheincreasingoccurrenceofA.baumanniiinfectionsinthehospitalcallsformeasurestorapidly characterize the isolates and identify emer