Dual Localized AtHscB Involved in Iron Sulfur Protein Biogenesis in Arabidopsis 英文参考文献.docVIP

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Dual Localized AtHscB Involved in Iron Sulfur Protein Biogenesis in Arabidopsis 英文参考文献.doc

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Dual Localized AtHscB Involved in Iron Sulfur Protein Biogenesis in Arabidopsis 英文参考文献

DualLocalizedAtHscBInvolvedinIronSulfurProtein BiogenesisinArabidopsis XiangMingXu,HongLin,MaitaLatijnhouwers,SimonGeirM?ller* CenterofOrganelleResearch,FacultyofScienceandTechnology,UniversityofStavanger,Stavanger,Norway Abstract Background:Iron-sulfurclustersareubiquitousstructureswhichactasprostheticgroupsfornumerousproteinsinvolvedin severalfundamentalbiologicalprocessesincludingrespirationandphotosynthesis.Althoughsimpleinstructureboththe assembly and insertion of clusters into apoproteins requires complex biochemical pathways involving a diverse set of proteins.Inyeast,theJ-typechaperoneJac1playsakeyroleinthebiogenesisofironsulfurclustersinmitochondria. Methodology/Principal Findings: Inthis study wedemonstrate thatAtHscB from Arabidopsis canrescue the Jac1 yeast knockoutmutantsuggestingaroleforAtHscBinironsulfurproteinbiogenesisinplants.IncontrasttomitochondrialJac1, AtHscBlocalizestobothmitochondriaandthecytosol.AtHscBinteractswithAtIscU1,anIsu-likescaffoldproteininvolvedin iron-sulfurclusterbiogenesis,andthroughthisinteractionAtIscU1ismostprobablyretainedinthecytosol.Thechaperone AtHscAcanfunctionallycomplementtheyeastSsq1knockoutmutantanditsATPaseactivityisenhancedbyAtHscBand AtIscU1. Interestingly, AtHscA is also localized in both mitochondria and the cytosol. Furthermore, AtHscB is highly expressedinanthersandtrichomesandanAtHscBT-DNAinsertionmutantshowsreducedseedset,awaxlessphenotype andinappropriatetrichomedevelopmentaswellasdramaticallyreducedactivitiesoftheiron-sulfurenzymesaconitaseand succinatedehydrogenase. Conclusions:OurdatasuggestthatAtHscBtogetherwithAtHscAandAtIscU1playsanimportantroleinthebiogenesisof iron-sulfurproteinsinbothmitochondriaandthecytosol. Citation:XuXM,LinH,LatijnhouwersM,M?llerSG(2009)DualLocalizedAtHscBInvolvedinIronSulfurProteinBiogenesisinArabidopsis.PLoSONE4(10):e7662. doi:10.1371/journal.pone.0007662 Editor:EdwardNewbigin,UniversityofMelbourne,Australia ReceivedJuly14,2009;AcceptedOctober9,2009;PublishedOctober29,2009