Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice 英文参考文献.docVIP

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Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice 英文参考文献.doc

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Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice 英文参考文献

DynamicNear-InfraredOpticalImagingof2- DeoxyglucoseUptakebyIntracranialGliomaofAthymic Mice HelingZhou1,KateLuby-Phelps2,BruceE.Mickey3,AmynA.Habib4,RalphP.Mason1,DawenZhao1* 1DepartmentofRadiology,TheUniversityofTexasSouthwesternMedicalCenter,Dallas,Texas,UnitedStatesofAmerica,2DepartmentofCellBiology,TheUniversityof TexasSouthwesternMedicalCenter,Dallas,Texas,UnitedStatesofAmerica,3DepartmentofNeurologicalSurgery,TheUniversityofTexasSouthwesternMedicalCenter, Dallas,Texas,UnitedStatesofAmerica,4DepartmentofNeurology,TheUniversityofTexasSouthwesternMedicalCenter,Dallas,Texas,UnitedStatesofAmerica Abstract Background:Itisrecognizedthatcancercellsexhibithighlyelevatedglucosemetabolismcomparedtonon-tumorcells.We have applied in vivo optical imaging to study dynamic uptake of a near-infrared dye-labeled glucose analogue, 2- deoxyglucose(2-DG)byorthotopicgliomainamousemodel. Methodology and Principal Findings: The orthotopic glioma model was established by surgically implanting U87-luc gliomacellsintotherightcaudal nuclearareaofnudemice.Intracranial tumorgrowthwasmonitoredlongitudinallyby bioluminescence imaging and MRI. When tumor size reached .4 mm diameter, dynamic fluorescence imaging was performed after an injection of the NIR labeled 2-DG, IRDye800CW 2-DG. Real-time whole body images acquired immediatelyafteri.v.infusionclearlyvisualizedthenear-infrareddyecirculatingintovariousinternalorganssequentially. Dynamic fluorescence imaging revealed significantly higher signal intensity in the tumor side of the brain than the contralateralnormalbrain24hafterinjection(tumor/normalratio,TNR =2.860.7).Evenstrongercontrastwasachievedby removing the scalp (TNR =3.761.1) and skull (TNR =4.261.1) of the mice. In contrast, a control dye, IRDye800CW carboxylate,showedlittledifference(1.160.2).Exvivofluorescenceimagingperformedonultrathincryosections(20mm)of tumorbearingwholebrainrevealeddistincttumormargins.Microscopicimagingidentifiedcytoplasmiclocationsofthe2- DGdyein