E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development 英文参考文献.docVIP

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E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development 英文参考文献.doc

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E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development 英文参考文献

E-CadherinPromotesIncorporationofMouseEpiblast StemCellsintoNormalDevelopment SatoshiOhtsuka1,SatomiNishikawa-Torikai1,2,HitoshiNiwa1,2 * 1Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB),Kobe, Japan, 2CREST (Core Research for Evolutional ScienceandTechnology), Japan ScienceTechnologyAgency,Kawaguchi,Japan Abstract Mouseepiblaststemcells(mEpiSCs)arepluripotentstemcellsderivedfromepiblastsofpostimplantationmouseembryos. Theirpluripotencyisdistinctfromthatofmouseembryonicstemcells(mESCs)inseveralcellbiologicalcriteria.Oneofthe distinctions is thatmEpiSCscontribute either notatallor atmuchlower efficiencytochimeric embryosafter blastocyst injectioncomparedtomESCs.However,hereweshowedthatmEpiSCscanbeincorporatedintonormaldevelopmentafter blastocystinjectionbyforcedexpressionoftheE-cadherintransgenefor2daysinculture.Usingthisstrategy,mEpiSCsgave rise to live-born chimeras from 5% of the manipulated blastocysts. There were no obvious signs of reprogramming of mEpiSCstowardthemESC-likestateduringthe2daysafterinductionoftheE-cadherintransgene,suggestingthatmEpiSCs possesslatentabilitytointegrateintothenormaldevelopmentalprocessasitsorigin,epiblasts. Citation: Ohtsuka S, Nishikawa-Torikai S, Niwa H (2012) E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development. PLoS ONE7(9):e45220.doi:10.1371/journal.pone.0045220 Editor:QiangWu,NationalUniversityofSingapore,Singapore ReceivedJune25,2012;AcceptedAugust14,2012;PublishedSeptember18,2012 Copyright: ? 2012 Ohtsuka et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by the Research Program of Innovative Cell Biology by Innovative Technology (Cell Innovation) from the Ministry of Education,Culture,Sports,ScienceandTechnology(MEXT)ofJapanandRIKEN(CenterforDevelopmen