Ectopic Expression of MiR-125a Inhibits the Proliferation and Metastasis of Hepatocellular Carcinoma by Targeting MMP11 and VEGF 英文参考文献.docVIP
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Ectopic Expression of MiR-125a Inhibits the Proliferation and Metastasis of Hepatocellular Carcinoma by Targeting MMP11 and VEGF 英文参考文献
EctopicExpressionofMiR-125aInhibitstheProliferation
andMetastasisofHepatocellularCarcinomaby
TargetingMMP11andVEGF
QianBi1.,ShanhongTang1.,LinXia1.,RuiDu1,RuiFan1,LiucunGao1,JiangJin1,ShuhuiLiang1,
ZhengChen1,GuanghuiXu1,YongzhanNie1,KaichunWu1,JieLiu1,2,YongquanShi1*,JieDing1*,
DaimingFan1
1StateKeyLaboratoryofCancerBiology,XijingHospitalofDigestiveDiseases,FourthMilitaryMedicalUniversity,Xi’an,P.R.China,2DepartmentofDigestiveDiseases,
HuashanHospital,FudanUniversity,Shanghai,China
Abstract
Background:StudieshavebeenshownthatmiR-125aplaysanimportantroleincarcinogenesis,however,theroleofmiR-
125ainhepatocellularcarcinoma(HCC)remainselusive.
Methodology/Principal: Real time-PCR (qRT-PCR) was performed to test the significance of miR-125a in HCC. Ectopic
expressionofmiR-125awasusedtotesttheinfluencesofmiR-125aonproliferationandmetastasisofHCCcellsinvitroand
invivo.PredictedtargetgenesofmiR-125aweredeterminedbydual-luciferasereporting,qRT-PCR,andwesternblot(WB)
analyses.Thenimmunohistochemicalstaining(IHC)wasusedtodetecttheexpressionoftargetgenes,andthecorrelations
andprognosticvaluesofmiR-125aanditstargetgeneswerealsoinvestigated.
Conclusions/Significance: Decreased miR-125a was observed in both HCC tissues and cell lines, and associated with
patients’ aggressive pathologic features. Up-regulating miR-125a significantly inhibited the malignant phenotypes by
repressingtheexpressionofmatrixmetalloproteinase11(MMP11)andvascularendothelialgrowthfactorA(VEGF-A)both
invitroandinvivo.Furthermore,miR-125aexpressionwasinverselycorrelatedwithbothMMP11andVEGF-Aexpressionin
HCC tissues. Inhibiting miR-125a could increase both MMP11 and VEGF-A expression, and RNA interference targeting
MMP11orVEGF-AmRNAcouldrescuethelossofmiR-125afunctions.MiR-125ainhibitstheproliferationandmetastasisof
HCCbytargetingMMP11andVEGF-A.Up-regulationofmiR-125amightbeapromisingapproachandaprognosticmarker
forHCC.
Citation:BiQ,TangS,XiaL,DuR,FanR,etal.(2012)EctopicExpressionofMiR-125aInhibitstheProlife
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