Effect of Pullulan Nanoparticle Surface Charges on HSA Complexation and Drug Release Behavior of HSA-Bound Nanoparticles 英文参考文献.docVIP

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Effect of Pullulan Nanoparticle Surface Charges on HSA Complexation and Drug Release Behavior of HSA-Bound Nanoparticles 英文参考文献.doc

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Effect of Pullulan Nanoparticle Surface Charges on HSA Complexation and Drug Release Behavior of HSA-Bound Nanoparticles 英文参考文献

EffectofPullulanNanoparticleSurfaceChargesonHSA ComplexationandDrugReleaseBehaviorofHSA-Bound Nanoparticles XiaojunTao1,4,QiufangZhang2,KaiLing4,YicunChen3,WenzhiYang4,FenfeiGao3,GanggangShi1,3* 1Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China, 2Laboratory of Chinese Herbal Pharmacology, RenminHospital of Hubei University of Medicine, Shiyan, Hubei, China, 3Department of Pharmacology, Shantou University Medical College, Shantou, Guangdong, China, 4Institute of Biomedical Engineering, Chinese Academy of Medical Science Peking Union Medical College, The Key Laboratory of Biomedical MaterialofTianjin,Tianjin,People’sRepublicofChina Abstract Nanoparticle(NP)compositionssuchashydrophobicityandsurfacechargearevitaltodeterminethepresenceandamount of human serum albumin (HSA) binding. The HSA binding influences drug release, biocompatibility, biodistribution, and intercellulartraffickingofnanoparticles(NPs).Here,weprepared2kindsofnanomaterialstoinvestigateHSAbindingand evaluated drug release of HSA-bound NPs. Polysaccharides (pullulan) carboxyethylated to provide ionic derivatives were thenconjugatedtocholesterolgroupstoobtaincholesterol-modifiedcarboxyethylpullulan(CHCP).Cholesterol-modified pullulan(CHP)conjugatewassynthesizedwithasimilardegreeofsubstitutionofcholesterolmoietytoCHCP.CHCPformed self-aggregated NPs in aqueous solution with a spherical structure and zeta potential of 219.960.23mV, in contrast to 21.2160.12mVofCHPNPs.NPscouldquenchalbuminfluorescenceintensitywithmaximumemissionintensitygradually decreasing up to a plateau at 9 to 12h. Binding constants were 1.126105M21 and 0.706105M21 to CHP and CHCP, respectively,asdeterminedbyStern-Volmeranalysis.ThecomplexationbetweenHSAandNPswasagradualprocessdriven byhydrophobicforceandinhibitedbyNPsurfacechargeandshell-corestructure.HSAconformationwasalteredbyNPs withreductionofa-helicalcontent,dependingoninteractiontimeandparticlesurface