Effect of 1918 PB1-F2 Expression on Influenza A Virus Infection Kinetics 英文参考文献.docVIP

下载本文档

4
0
约5.53万字
约 12页
2017-05-11 发布于上海
举报
版权申诉

Effect of 1918 PB1-F2 Expression on Influenza A Virus Infection Kinetics 英文参考文献.doc

1、本文档共12页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Effect of 1918 PB1-F2 Expression on Influenza A Virus Infection Kinetics 英文参考文献

Effectof1918PB1-F2ExpressiononInfluenzaAVirus InfectionKinetics AmberM.Smith1,FrederickR.Adler2,JulieL.McAuley3,RyanN.Gutenkunst4,RuyM.Ribeiro1, JonathanA.McCullers5,AlanS.Perelson1* 1TheoreticalBiologyandBiophysics,LosAlamosNationalLaboratory,LosAlamos,NewMexico,UnitedStatesofAmerica,2DepartmentsofMathematicsandBiology, University of Utah, Salt Lake City, Utah, United States of America, 3Department of Immunology and Microbiology, University of Melbourne, Victoria, Australia, 4DepartmentofMolecularandCellularBiology,UniversityofArizona,Tucson,Arizona,UnitedStatesofAmerica,5DepartmentofInfectiousDiseases,St.JudeChildren’s ResearchHospital,Memphis,Tennessee,UnitedStatesofAmerica Abstract Relativelylittleisknownabouttheviralfactorscontributingtothelethalityofthe1918pandemic,althoughitsunparalleled virulence was likely due in part to the newly discovered PB1-F2 protein. This protein, while unnecessary for replication, increasesapoptosisinmonocytes,altersviralpolymeraseactivityinvitro,enhancesinflammationandincreasessecondary pneumonia in vivo. However, the effects the PB1-F2 protein have in vivo remain unclear. To address the mechanisms involved,weintranasallyinfectedgroupsofmicewitheitherinfluenzaAvirusPR8orageneticallyengineeredvirusthat expresses the 1918 PB1-F2 protein on a PR8 background, PR8-PB1-F2(1918). Mice inoculated with PR8 had viral concentrations peaking at 72hours, while those infected with PR8-PB1-F2(1918) reached peak concentrations earlier, 48hours.MicegivenPR8-PB1-F2(1918)alsoshowedafasterdeclineinviralloads.Wefitamathematicalmodeltothese datatoestimateparametervalues.Themodelsupportsahigherviralproductionratepercellandahigherinfectedcell deathratewiththePR8-PB1-F2(1918)virus.Wediscusstheimplicationsthesemechanismshaveduringaninfectionwitha virusexpressingavirulentPB1-F2onthepossibilityofapandemicandontheimportanceofantiviraltreatments. Citation:SmithAM,AdlerFR,McAuleyJL,GutenkunstRN,RibeiroRM,etal.(2011)Effectof1918PB1-F2ExpressiononInfluenzaAVirus