Expression of Kruppel-Like Factor KLF4 in Mouse Hair Follicle Stem Cells Contributes to Cutaneous Wound Healing 英文参考文献.docVIP

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Expression of Kruppel-Like Factor KLF4 in Mouse Hair Follicle Stem Cells Contributes to Cutaneous Wound Healing 英文参考文献.doc

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Expression of Kruppel-Like Factor KLF4 in Mouse Hair Follicle Stem Cells Contributes to Cutaneous Wound Healing 英文参考文献

ExpressionofKruppel-LikeFactorKLF4inMouseHair FollicleStemCellsContributestoCutaneousWound Healing JuanLi1,2.,HaiZheng3.,JunfengWang1,2,FangYu1,RebeccaJ.Morris4,TimothyC.Wang5, ShiangHuang2*,WaldenAi1* 1DepartmentofPathology,MicrobiologyandImmunology,UniversityofSouthCarolinaSchoolofMedicine,Columbia,SouthCarolina,UnitedStatesofAmerica,2Centre forStemCellResearchandApplication,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan,China,3DepartmentofGeneral Surgery,Union Hospital,TongjiMedical College, HuazhongUniversity ofScienceand Technology, Wuhan, China, 4Laboratory ofStem Cells and Cancer,TheHormel Institute,University ofMinnesota, Austin, Minnesota, United States ofAmerica, 5Division of Digestive and Liver Diseases, Department of Medicine and Irving Cancer Center,ColumbiaUniversity,NewYork,NewYork,UnitedStatesofAmerica Abstract Background:Kruppel-likefactorKLF4isatranscriptionfactorcriticalfortheestablishmentofthebarrierfunctionoftheskin. Itsfunctioninstemcellbiologyhasbeenrecentlyrecognized.Previousstudieshaverevealedthathairfolliclestemcells contributetocutaneouswoundhealing.However,expressionofKLF4inhairfolliclestemcellsandtheimportanceofsuch expressionincutaneouswoundhealinghavenotbeeninvestigated. Methodology/PrincipalFindings:Quantitativerealtimepolymerasechainreaction(RT-PCR)analysisshowedhigherKLF4 expressioninhairfolliclestemcell-enrichedmouseskinkeratinocytesthanthatincontrolkeratinocytes.WegeneratedKLF4 promoter-driven enhanced green fluorescence protein (KLF4/EGFP) transgenic mice and tamoxifen-inducible KLF4 knockoutmicebycrossingKLF4promoter-drivenCrerecombinasefusedwithtamoxifen-inducibleestrogenreceptor(KLF4/ CreERTM) transgenic mice with KLF4(flox) mice. KLF4/EGFP cells purified from dorsal skin keratinocytes of KLF4/EGFP transgenicmicewereco-localizedwith5-bromo-2’-deoxyuridine(BrdU)-labelretainingcellsbyflowcytometricanalysisand immunohistochemistry. Lineage tracing was performed in the context of cutaneous w