Expression of Baculovirus Anti-Apoptotic Genes p35 and op-iap in Cotton (Gossypium hirsutum L.) Enhances Tolerance to Verticillium Wilt 英文参考文献.docVIP

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Expression of Baculovirus Anti-Apoptotic Genes p35 and op-iap in Cotton (Gossypium hirsutum L.) Enhances Tolerance to Verticillium Wilt 英文参考文献.doc

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Expression of Baculovirus Anti-Apoptotic Genes p35 and op-iap in Cotton (Gossypium hirsutum L.) Enhances Tolerance to Verticillium Wilt 英文参考文献

ExpressionofBaculovirusAnti-ApoptoticGenesp35and op-iapinCotton(GossypiumhirsutumL.)Enhances TolerancetoVerticilliumWilt JuanTian1.,XueyanZhang2.,BenguoLiang3,ShanweiLi4,ZhixiaWu2,QianhuaWang2,ChunxuLeng1, JiangliDong1*,TaoWang1 1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China, 2Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China, 3Horticulture Department, Xinyang AgriculturalCollege,Xinyang,China,4StateKeyLaboratoryofCropBiology,ShandongAgriculturalUniversity,Taian,China Abstract Background:Programmedcelldeathplaysanimportantroleinmediatingplantadaptiveresponsestotheenvironment suchastheinvasionofpathogens.Verticilliumwilt,causedbythenecrotrophicpathogenVerticilliumdahliae,isaserious vasculardiseaseresponsibleforgreateconomiclossestocotton,butthemolecularmechanismsofverticilliumdiseaseand effective,safemethodsofresistancetoverticilliumwiltremainunexplored. Methodology/PrincipalFindings:Inthisstudy,weintroducedbaculovirusapoptosisinhibitorgenesp35andop-iapinto the genome of cotton via Agrobacterium-mediated transformation and analyzed the response of transgenic plants to verticilliumwilt.Resultsshowedthatp35andop-iapconstructswerestablyintegratedintothecottongenome,expressedin thetransgeniclines,andinheritedthroughtheT3generation.Thetransgeniclineshadsignificantlyincreasedtoleranceto verticillium wilt throughout the developmental stages. The disease index of T1–T3 generation was lower than 19, significantly(P,0.05)betterthanthenegativecontrollinez99668.Aftertreatmentwith250mg/LVD-toxinsfor36hours, DNAfromnegativecontrolleaveswasfragmented,whereasfragmentationinthetransgenicleafDNAdidnotoccur.The percentageofcelldeathintransgeniclinesincreasedby7.11%after60mg/LVD-toxintreatment,whichwaslessthanthat ofthenegativecontrollines’s21.27%.Thisindicatesthatp35andop-iapgeneexpressionpartiallyprotect