Expression of Pseudomonas aeruginosa CupD Fimbrial Genes Is Antagonistically Controlled by RcsB and the EAL-Containing PvrR Response Regulators 英文参考文献.docVIP

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Expression of Pseudomonas aeruginosa CupD Fimbrial Genes Is Antagonistically Controlled by RcsB and the EAL-Containing PvrR Response Regulators 英文参考文献.doc

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Expression of Pseudomonas aeruginosa CupD Fimbrial Genes Is Antagonistically Controlled by RcsB and the EAL-Containing PvrR Response Regulators 英文参考文献

ExpressionofPseudomonasaeruginosaCupDFimbrial GenesIsAntagonisticallyControlledbyRcsBandthe EAL-ContainingPvrRResponseRegulators HelgaMikkelsen1,Genevie`veBall2,CarolineGiraud2,AlainFilloux1,2* 1ImperialCollegeLondon,DivisionofCellandMolecularBiology,CentreforMolecularMicrobiologyandInfection,SouthKensingtonCampus,London,UnitedKingdom, 2Laboratoired’Inge′nierie desSyste`mesMacromole′culaires, UPR9027,CNRS-IBSM,Marseille,France Abstract Pseudomonasaeruginosaisagram-negativepathogenicbacteriumwithahighadaptivepotentialthatallowsproliferation inabroadrangeofhostsorniches.Itisalsothecausativeagentofbothacuteandchronicbiofilm-relatedinfectionsin humans.Threecupgeneclusters(cupA-C),involvedintheassemblyofcellsurfacefimbriae,havebeenshowntobeinvolved inbiofilmformationbytheP.aeruginosastrainsPAO1orPAK.InPA14isolates,afourthcluster,namedcupD,wasidentified withinapathogenicityisland,PAPI-I,andmaycontributetothehighervirulenceofthisstrain.ExpressionofthecupAgenes iscontrolledbytheHNS-likeproteinMvaT,whereasthecupBandcupCgenesareunderthecontroloftheRocS1A1Rtwo- componentsystem. Inthisstudy, weshowthat cupD geneexpressionis positively controlled bytheresponse regulator RcsB.Asaconsequence,CupDfimbriaeareassembledonthecellsurface,whichresultsinanumberofphenotypessuchas asmallcolonymorphotype,increasedbiofilmformationanddecreasedmotility.Thesebehaviorsarecompatiblewiththe sessile bacterial lifestyle. The balance between planktonic and sessile lifestyles is known to be linked to the intracellular levelsofc-di-GMPwithhighlevelsfavoringbiofilmformation.WeshowedthattheEALdomain-containingPvrRresponse regulator counteracts the activity of RcsB on cupD gene expression. The action of PvrR is likely to involve c-di-GMP degradationthroughphosphodiesteraseactivity,confirmingthekeyroleofthissecondmessengerinthebalancebetween bacteriallifestyles.TheregulatorynetworkbetweenRcsBandPvrRremainstobeelucidated,butitstandsasapotential modelsystemtostudyhowtheequilibriumbetweenthetwolifestylesc