Extensive In Vivo Resilience of Persistent Salmonella 英文参考文献.docVIP

ExtensiveInVivoResilienceofPersistentSalmonella SomeduttaBarat,BenjaminSteeb,AlainMaze′,DirkBumann* FocalAreaInfectionBiology,Biozentrum,UniversityofBasel,Basel,Switzerland Abstract Chronic infections caused by persistent pathogens represent an important health problem. Here, we establish a simple practical mouse Salmonella infection model for identifying bacterial maintenance functions that are essential for persistency. In this model, a substantial fraction of Salmonella survived even several days of treatment with a potent fluoroquinoloneantibioticindicatingstringencyofthemodel.Evaluationoftwelvemetabolicdefectsrevealeddramatically different requirements for Salmonella during persistency as compared to acute infections. Disrupted synthesis of unsaturated/cyclopropanefattyacidswastheonlydefectthatresultedinrapidSalmonellaclearancesuggestingthatthis pathwaymightcontainsuitabletargetsforantimicrobialchemotherapyofchronicinfection. Citation: Barat S, Steeb B, Maze′ A, Bumann D (2012) Extensive In Vivo Resilience of Persistent Salmonella. PLoS ONE 7(7): e42007. doi:10.1371/ journal.pone.0042007 Editor:MichaelHensel,UniversityofOsnabrueck,Germany ReceivedFebruary7,2012;AcceptedJuly2,2012;PublishedJuly24,2012 Copyright: ? 2012 Barat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbyDeutscheForschungsgemeinschaft(EuropeanResearchTrainingGroup1273)andSchweizerischerNationalfonds(31003A- 121834).Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:Dirk.Bumann@unibas.ch Introduction recommendedfortreatmentofchronicSalmonellatissuecoloniza- tion[15]. Persistent pathogens represent a major problem for control of infectiousdiseases[1].Ext