Fast Benchtop Fabrication of Laminar Flow Chambers for Advanced Microscopy Techniques 英文参考文献.docVIP

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Fast Benchtop Fabrication of Laminar Flow Chambers for Advanced Microscopy Techniques 英文参考文献.doc

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Fast Benchtop Fabrication of Laminar Flow Chambers for Advanced Microscopy Techniques 英文参考文献

FastBenchtopFabricationofLaminarFlowChambersfor AdvancedMicroscopyTechniques DavidS.Courson,RonaldS.Rock* DepartmentofBiochemistryandMolecularBiology,TheUniversityofChicago,Chicago,Illinois,UnitedStatesofAmerica Abstract Background:Fluidhandlingtechnologyisacquiringanevermoreprominentplaceinlaboratorysciencewhetheritisin simplebufferexchangesystems,perfusionchambers,oradvancedmicrofluidicdevices.Manyoftheseapplicationsremain theprovidenceoflaboratoriesatlargeinstitutionswithagreatdealofexpertiseandspecializedequipment.Evenwiththe expansionofthesetechniques,limitationsremainthatfrequentlypreventthecouplingofcontrolledfluidflowwithother technologies, such as coupling microfluidics and high-resolution position and force measurements by optical trapping microscopy. Method:Herewepresentamethodforfabricationofmultiple-inputlaminarflowdevicesthatareopticallyclear[glass]on each face, chemically inert, reusable, inexpensive, and can be fabricated on the benchtop in approximately one hour. Further these devices are designed to allow flow regulation by a simple gravity method thus requiring no specialized equipment to drive flow. Here we use these devices to perform total internal reflection fluorescence microscopy measurementsaswellaspositionsensitiveopticaltrappingexperiments. Significance: Flow chamber technology needs to be more accessible to the general scientific community. The method presented here is versatile and robust. These devices use standard slides and coverslips making them compatible with nearlyalltypesandmodelsoflightmicroscopes.Thesedevicesmeettheneedsofgroupsdoingadvancedopticaltrapping experiments,butcouldalsobeadaptedbynearlyanylabthathasafunctionforsolutionflowcoupledwithmicroscopy. Citation: Courson DS, Rock RS (2009) Fast Benchtop Fabrication of Laminar Flow Chambers for Advanced Microscopy Techniques. PLoS ONE 4(8): e6479. doi:10.1371/journal.pone.0006479 Editor:ReinUlijn,UniversityofStrathclyde,UnitedKingdom ReceivedMarch24,2009;AcceptedJune19,2009