Expression Profiling of MAP Kinase–Mediated Meiotic Progression in Caenorhabditis elegans 英文参考文献.docVIP

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Expression Profiling of MAP Kinase–Mediated Meiotic Progression in Caenorhabditis elegans 英文参考文献.doc

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Expression Profiling of MAP Kinase–Mediated Meiotic Progression in Caenorhabditis elegans 英文参考文献

ExpressionProfilingofMAPKinase– MediatedMeioticProgression inCaenorhabditiselegans Stefanie W. Leacock, Valerie Reinke* DepartmentofGenetics,YaleUniversitySchoolofMedicine,NewHaven,Connecticut,UnitedStatesofAmerica TheLET-60(Ras)/LIN-45(Raf)/MPK-1(MAPkinase)signalingpathwayplaysakeyroleinthedevelopmentofmultiple tissuesinCaenorhabditiselegans.Forthemostpart,theidentitiesofthedownstreamgenesthatactastheultimate effectors of MPK-1 signaling have remained elusive. A unique allele of mpk-1, ga111, displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111)animals toidentify candidate downstream effectorsof MPK-1signaling in the germline.ThisanalysisdelineatedacohortofgeneswhoseexpressionrequiresMPK-1signalingingermcellsinthe pachytenestageofmeiosisI.RNAinsituhybridizationanalysisshowsthatthesegenesareexpressedinthegermline inanMPK-1-dependentmannerandhaveaspatialexpressionpatternconsistentwiththelocationofactivatedMPK-1. We found that one MPK-1 signaling-responsive gene encoding a C2H2 zinc finger protein plays a role in meiotic chromosome segregation downstream of MPK-1. Additionally, discovery of genes responsive to MPK-1 signaling permittedustoorderMPK-1signalingrelativetoseveraleventsoccurringinpachytene,includingEFL-1/DPL-1gene regulation and X chromosome reactivation. This study highlights the utility of applying global gene expression methodstoinvestigategenesdownstreamofcommonlyusedsignalingpathwaysinvivo. Citation: Leacock SW, ReinkeV (2006) Expression profiling of MAP kinase–mediated meiotic progression in Caenorhabditis elegans. PLoS Genet 2(11): e174. doi:10.1371/ journal.pgen.0020174 protein required for proper meiotic progression and oo- genesis, is also present during pachytene and prevents Introduction Signal transduction pathways play key roles in specifying cell fates. Most signaling pathways terminate in the nucleus andaltertheexpressionofas