Gene Expression Profiling Reveals New Potential Players of Gonad Differentiation in the Chicken Embryo 英文参考文献.docVIP

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Gene Expression Profiling Reveals New Potential Players of Gonad Differentiation in the Chicken Embryo 英文参考文献.doc

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Gene Expression Profiling Reveals New Potential Players of Gonad Differentiation in the Chicken Embryo 英文参考文献

GeneExpressionProfilingRevealsNewPotentialPlayers ofGonadDifferentiationintheChickenEmbryo Gwenn-Ae¨lCarre′1,IsabelleCouty1,ChristelleHennequet-Antier2,MarinaS.Govoroun1* 1PhysiologiedelaReproductionetdesComportementsUMR6175,INRA-CNRS-Universite′ F.RabelaisdeTours-HarasNationaux,Nouzilly,France,2Unite′ deRecherche Avicole,INRA,Nouzilly,France Abstract Background:Inbirdsasinmammals,ageneticswitchdetermineswhethertheundifferentiatedgonaddevelopsintoan ovaryoratestis.However,understandingofthemolecularpathway(s)involvedingonaddifferentiationisstillincomplete. Methodology/Principal Findings: With the aim of improving characterization of the molecular pathway(s) involved in gonaddifferentiationinthechickenembryo,wedevelopedalargescalerealtimereversetranscriptionpolymerasechain reactionapproachon110selectedgenesforevaluationoftheirexpressionprofilesduringchickengonaddifferentiation betweendays5.5and19ofincubation.Hierarchicalclusteringanalysisoftheresultingdatasetsdiscriminatedgeneclusters expressedpreferentiallyintheovaryorthetestis,and/oratearlyorlaterperiodsofembryonicgonaddevelopment.Fitting a linear model and testing the comparisons of interest allowed the identification of new potential actors of gonad differentiation,suchasZ-linkedADAMTS12,LOC427192(correspondingtoNIM1protein)andCFC1,thatareupregulatedin the developing testis, and BMP3 and Z-linked ADAMTSL1, that are preferentially expressed in the developing ovary. Interestingly, the expression patterns of several members of the transforming growth factor b family were sexually dimorphic,withinhibinsubunitsupregulatedinthetestis,andbonemorphogeneticproteinsubfamilymembersincluding BMP2,BMP3,BMP4andBMP7,upregulatedintheovary.Thisstudyalsohighlightedseveralgenesdisplayingasymmetric expressionprofilessuchasGREM1andBMP3thatarepotentiallyinvolvedindifferentaspectsofgonadleft-rightasymmetry. Conclusion/Significance:Thisstudysupportstheoverallconservationofvertebratesexdifferentiationpathwaysbutalso revealssome