Genetic Characterization of Trypanosoma cruzi DTUs in Wild Triatoma infestans from Bolivia Predominance of TcI 英文参考文献.docVIP
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Genetic Characterization of Trypanosoma cruzi DTUs in Wild Triatoma infestans from Bolivia Predominance of TcI 英文参考文献
GeneticCharacterizationofTrypanosomacruziDTUsin
WildTriatomainfestansfromBolivia:Predominanceof
TcI
SimoneFre′de′riqueBrenie`re1,2*,ClaudiaAliaga1,2,EtienneWaleckx1,2,RosioBuitrago1,2,RenataSalas1,2
,
ChristianBarnabe′1,2,MichelTibayrenc1,Franc?ois Noireau1,3{
1MIVEGEC(Universite′deMontpellier1et2,CNRS5290,IRD224),MaladiesInfectieusesetVecteurs:Ecologie,Ge′ne′tique,EvolutionetContro?le,InstitutdeRecherchepour
lede′veloppement (IRD),RepresentationinBolivia,LaPaz,Bolivia,2InstitutoNacionaldeLaboratoriosdeSalud(INLASA),LaboratoriodeEntomologiaMe′dica, LaPaz,
Bolivia,3IIBISMED,FacultaddeMedicina,UniversidadMayordeSanSimo′n,Cochabamba,Bolivia
Abstract
Background:ThecurrentpersistenceofTriatomainfestans(oneofthemainvectorsofChagasdisease)insomedomestic
areascouldberelatedtore-colonizationbywildpopulationswhichareincreasinglyreported.However,theinfectionrate
andthegeneticcharacterizationoftheTrypanosomacruzistrainsinfectingthesepopulationsareverylimited.
Methodology/Principal Findings: Of 333 wild Triatoma infestans specimens collected from north to south of a Chagas
diseaseendemicareainBolivia,wecharacterized234stocksofTrypanosomacruziusingmini-exonmultiplexPCR(MMPCR)
andsequencingtheglucosephosphateisomerase(Gpi)gene.Ofthesixgeneticlineages(‘‘discretetypingunits’’;DTU)(TcI-
VI) presently recognized in T. cruzi, TcI (99.1%) was overdominant on TcIII (0.9%) in wild Andean T. infestans , which
presenteda71.7%infectionrateasevaluatedbymicroscopy.Inthelowlands(BolivianChaco),17‘‘darkmorph’’T.infestans
wereanalyzed.Noneofthemwerepositiveforparasitesaftermicroscopicexamination,althoughoneTcIstockandoneTcII
stockwereidentifiedusingMMPCRandsequencing.
Conclusions/Significance:Byexploringlarge-scaleDTUsthatinfectthewildpopulationsofT.infestans,thisstudyopensthe
discussionontheoriginofTcIandTcVDTUsthatarepredominantindomesticBoliviancycles.
Citation:Brenie`reSF,AliagaC,WaleckxE,BuitragoR,SalasR,etal.(2012)GeneticCharacterizationofTrypanosomacruziDTUsinWildTriatomainfe
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