HCV Subtype Characterization among Injection Drug Users Implication for a Crucial Role of Zhenjiang in HCV Transmission in China 英文参考文献.docVIP

HCVSubtypeCharacterizationamongInjectionDrug Users:ImplicationforaCrucialRoleofZhenjianginHCV TransmissioninChina ChiyuZhang1*,NanaWu1,JunLiu1,QinjuanGe2,YanHuang1,QianRen1,QingchuanFeng1,3 ,Guangli He2 1InstituteofLifeSciences,JiangsuUniversity,Zhenjiang,Jiangsu,China,2ZhenjiangCenterofDiseaseControlandPrevention,Zhenjiang,Jiangsu,China,3Department ofCellBiologyandMedicineGenetics,CollegeofBasicMedicine,ZhengzhouUniversity,Zhengzhou,Henan,China Abstract Background:HCVtransmissioniscloselyassociatedwithdrug-traffickingroutesinChina.However,thetransmissionroute ofHCVinEasternChinaremainsunclear.Here,weinvestigatetheroleofZhenjiangcityofJiangsuprovince,animportant transportationhublinkingShanghaiwithotherregionsofChina,inHCVtransmission. Methodology/PrincipalFindings:Atotalof141wholebloodsampleswerecollectedfrominjectiondrugusers(IDUs)in ZhenjiangandthentestedforHCVinfection.Ofthem,115HCVpositiveplasmasweresubjectedtoRNAextraction,RT-PCR amplification, and sequencing. The subtype characterization and the evolutionary origin of HCV strains circulating in Zhenjiangweredeterminedusingpolygeneticorphylogeographicanalyses.SevenHCVsubtypes1b,2a,3a,3b,6a,6eand 6n were detected among Zhenjiang IDUs, showing a complex HCV epidemic. The most predominant subtypes were 3a (38%)and1b(26.8%).Amongthesesubtypes,subtypes3b,6nand6eoriginatedfromSouthwesternChina(i.e.,Yunnan and/or Guangxi), subtypes 2a and 6a from Southern China (i.e., Guangdong), subtype 1b from Central (i.e., Henan) and Northwestern(i.e.,Xinjiang)China,andsubtype3afromSouthwestern(i.e.,Yunnan)andNorthwestern(i.e.,Xinjiang)China. From Zhenjiang, subtypes 1b and 2a were further spread to Eastern (i.e., Shanghai) and Northern (i.e., Beijing) China, respectively. Conclusions/Significance:ThemixingofsevenHCVsubtypesinZhenjiangfromallquartersofChinaindicatesthatasan importantmiddlestation,ZhenjiangplaysacrucialroleinHCVtransmission,justasitisimportantinpopulationmigration betweenotherregionsofChinaandEasternChina. Citat