海滨土壤芽胞杆菌的初步鉴定及其次级代谢产物的性质.pdfVIP

申咽 i鹏柑九 f 学报 368 Joumal of China Phannaceutical Uníversity 2∞8 ， 39(4) :368 叩 372 海滨土壤芽胞杆菌的初步鉴定及其次级代谢产物的性质 杨烨， 臭\张婷婷，蔡寅，毕然，罗晨 (中国药科大学生命科学与技术学院，南京 21∞09) 摘要 目的:对从黄海海滨土壤中分离得到的简株进行鉴定并研究其次级代谢产物的抗菌、抗肿瘤活性和结构信 息。方法:分析分离得到的商株的 168 rDNA、形态等特征，确定其种属以及研究其次级代俯产物(BSC) 的抗菌抗肿瘤治性， 并对 BSC 的结构进行了初步分析。结果:根据该株菌的 168 rDNA、形态等特征，确定其为1胞杆菌属，其次级代谢产物 BSC 具有一定的抗篱抗肿瘤治性，其分子式为 C哺H剧。190 结论:分离得到的菌株为芽胞杆菌i碍，其次级代谢产物 BSC 具有卢定 的抗筒、抗肿痛活性，是潜在的抗生素类药物。 关键诩 穿胞杆简;次级代谢产物;抗菌:抗肿瘤;结构分析 中圄分提号 Q939.1 文献标识码 A 烹章编号 1删叩5048(2∞8)04 -0368 -05 Identification of Bacillus sβCPU5 and characteristics of its secondary metabφ liteyANG Ye , WANG Min , ZHANG Ting-ti吨， CAI Yin , BI Ran , LUO Chen School of li/e Science arui Techrwlogy, China p阳， mωceωical University, Nanjir啄 21α脚， China Abstract Aim: To identify the isolated strain from the soil sample obtained near the yellow sea beach and to in嗣 vestigate the antibacterial and antitumor activities of its secondary metabolite 嗣 well 捕阳 structural infonnation. Methods: 币le 16s r DNA and morphological characteristics of the isolated strain were investigated. BSC，由e sec- ondary metabolite of the strain , w踊 also studied for its antibacterial and antitumor activities , as well 邸 i阳 struc­ ture. Results: Based on the 16s rDNA and morphological features , this new s位am w拙 confinned to be Bacillus sp. , BSC effectively inhibited the growth of bacterial and tumor cells in vitro , and the molecular fonnula of BSC is C H 0 , Conclusion: 币le isolated strain belongs to Bacülus Sp. BSC h嗣 fair antibacterial and antitumor 48 94 19 activities , and it is one potential antibiotic. Key words Bαcillus Sp. ; secondary metabolite; antibacterial; antitumor; structure analysis This sludy wωsupported， by tbe Sixth Talent Peak Project of Jiangsu 民uvince