MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway 英文参考文献.docVIP

下载本文档

9
0
约4.33万字
约 11页
2017-05-12 发布于上海
举报
版权申诉

MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway 英文参考文献

MCPIP1Down-RegulatesIL-2ExpressionthroughanARE- IndependentPathway MinLi1.,WenqiangCao1.,HaifengLiu2,WeiZhang1,XiaLiu1,ZhijianCai1,JingGuo1,XuelianWang1, ZhaoyuanHui1,HangZhang1,JianliWang1*,LieWang1* 1InstituteofImmunology,ZhejiangUniversitySchoolofMedicine,Hangzhou,China,2LaboratoryofMolecularCellBiology,InstituteofBiochemistryandCellBiology, ChineseAcademyofSciences,Shanghai,China Abstract IL-2playsakeyroleinthesurvivalandproliferationofimmunecells,especiallyTlymphocytes.Itsexpressionisprecisely regulatedattranscriptionalandposttranscriptionallevel.IL-2isknowntoberegulatedbyRNAbindingproteins,suchas tristetraprolin(TTP),viaanAU-richelement(ARE)inthe39-untranslatedregion(39UTR)toinfluencethestabilityofmRNA. MCPIP1, identified as a novel RNase, can degrade IL-6, IL-12 and TNF-a mRNA by an ARE-independent pathway in the activationofmacrophages.Here,wereportedthatMCPIP1wasinducedintheactivationofTlymphocytesandnegatively regulatedIL-2geneexpressioninbothmouseandhumanprimaryTlymphocytesthroughdestabilizingitsmRNA.Asetof Luciferase reporter assay demonstrated that a non-ARE conserved element in IL-2 39UTR, which formed a stem-loop structure,respondedtoMCPIP1activity.RNAimmunoprecipitationandBiotinpulldownexperimentsfurthersuggestedthat MCPIP1couldmodestlybindtoIL-2mRNA.Takentogether,thesedatademonstratethatMCPIP1down-regulatesIL-2viaan ARE-independentpathway. Citation: Li M, Cao W, Liu H, Zhang W, Liu X, et al. (2012) MCPIP1 Down-Regulates IL-2 Expression through an ARE-Independent Pathway. PLoS ONE 7(11): e49841.doi:10.1371/journal.pone.0049841 Editor:GeorgStoecklin,GermanCancerResearchCenter,Germany ReceivedApril22,2012;AcceptedOctober12,2012;PublishedNovember21,2012 Copyright:?2012Lietal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbygrantsfromtheNationalNaturalScienceFoundationo