Membrane Protein Location-Dependent Regulation by PI3K (III) and Rabenosyn-5 in Drosophila Wing Cells 英文参考文献.docVIP

MembraneProteinLocation-DependentRegulationby PI3K(III)andRabenosyn-5inDrosophilaWingCells MasatoAbe1¤a,YukaSetoguchi1,TsubasaTanaka3¤b,WakaeAwano2,KuniakiTakahashi4,RyuUeda4, AkiraNakamura3,SatoshiGoto1,5* 1GlycobiologyandGlycotechnologyResearchGroup,MitsubishiKagakuInstituteofLifeSciences,Tokyo,Japan,2MutantFliesLaboratory,MitsubishiKagakuInstituteof LifeSciences,Tokyo,Japan,3LaboratoryforGermlineDevelopment,RIKENCenterforDevelopmentBiology,Hyogo,Japan,4InvertebrateGeneticsLaboratory,National InstituteofGenetics,Shizuoka,Japan,5PrecursoryResearchforEmbryonicScienceandTechnology(PRESTO),JapanScienceandTechnologyAgency,Saitama,Japan Abstract TheclassIIIphosphatidylinositol-3kinase(PI3K(III))regulatesintracellularvesiculartransportatmultiplestepsthroughthe productionofphosphatidylinositol-3-phosphate(PI(3)P).Whilethelocalizationofproteinsatdistinctmembranedomains are likely regulated in different ways, the roles of PI3K (III) and its effectors have not been extensively investigated in a polarizedcellduringtissuedevelopment.Inthisstudy,weexaminedinvivofunctionsofPI3K(III)anditseffectorcandidate Rabenosyn-5(Rbsn-5)inDrosophilawingprimordialcells,whicharepolarizedalongtheapical-basalaxis.Knockdownofthe PI3K(III)subunitVps15resultedinanaccumulationoftheapicaljunctionalproteinsDE-cadherinandFlamingoandalsothe basalmembraneproteinb-integrininintracellularvesicles.Bycontrast,knockdownofPI3K(III)increasedlateralmembrane- localized Fasciclin III (Fas III). Importantly, loss-of-function mutation of Rbsn-5 recapitulated the aberrant localization phenotypes of b-integrin and Fas III, but not those of DE-cadherin and Flamingo. These results suggest that PI3K (III) differentiallyregulateslocalizationofproteinsatdistinctmembranedomainsandthatRbsn-5mediatesonlyapartofthe PI3K(III)-dependentprocesses. Citation:AbeM,SetoguchiY,TanakaT,AwanoW,TakahashiK,etal.(2009)MembraneProteinLocation-DependentRegulationbyPI3K(III)andRabenosyn-5in DrosophilaWingCells.PLoSONE4(10):e7306.doi:10.1371