Meiosis-Specific Loading of the Centromere-Specific Histone CENH3 in Arabidopsis thaliana 英文参考文献.docVIP

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Meiosis-Specific Loading of the Centromere-Specific Histone CENH3 in Arabidopsis thaliana 英文参考文献.doc

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Meiosis-Specific Loading of the Centromere-Specific Histone CENH3 in Arabidopsis thaliana 英文参考文献

Meiosis-SpecificLoadingoftheCentromere-Specific HistoneCENH3inArabidopsisthaliana MaruthachalamRavi1,FukashiShibata2,JosephS.Ramahi1,KiyotakaNagaki2,ChangbinChen3 ,Minoru Murata2,SimonW.L.Chan1* 1DepartmentofPlantBiology,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica,2InstituteofPlantScienceandResources,OkayamaUniversity, Kurashiki,Japan,3DepartmentofHorticulturalScience,UniversityofMinnesota,St.Paul,Minnesota,UnitedStatesofAmerica Abstract CentromerebehaviorisspecializedinmeiosisI,sothatsisterchromatidsofhomologouschromosomesarepulledtoward thesamesideofthespindle(throughkinetochoremono-orientation)andchromosomenumberisreduced.Factorsrequired formono-orientationhavebeenidentifiedinyeast.However,comparativelylittleisknownabouthowmeioticcentromere behavior is specialized in animals and plants that typically have large tandem repeat centromeres. Kinetochores are nucleated by the centromere-specific histone CENH3. Unlike conventional histone H3s, CENH3 is rapidly evolving, particularlyinitsN-terminaltaildomain.HerewedescribechimericvariantsofCENH3withalterationsintheN-terminaltail that are specifically defective in meiosis. Arabidopsis thaliana cenh3 mutants expressing a GFP-tagged chimeric protein containingtheH3N-terminaltailandtheCENH3C-terminus(termedGFP-tailswap)aresterilebecauseofrandommeiotic chromosome segregation. These defects result from the specific depletion of GFP-tailswap protein from meiotic kinetochores,whichcontrastswithitsnormallocalizationinmitoticcells.LossoftheGFP-tailswapCENH3variantinmeiosis affectsrecruitmentoftheessentialkinetochoreproteinMIS12.OurfindingssuggestthatCENH3loadingdynamicsmightbe regulateddifferentlyinmitosisandmeiosis.Asfurthersupportforourhypothesis,weshowthatGFP-tailswapproteinis recruited back to centromeres in a subset of pollen grains in GFP-tailswap once they resume haploid mitosis. Meiotic recruitmentoftheGFP-tailswapCENH3variantisnotrestoredbyremovalofthemeiosis-specificcohesinsubunitREC8.Our results