No Gold Standard Estimation of the Sensitivity and Specificity of Two Molecular Diagnostic Protocols for Trypanosoma brucei spp. in Western Kenya 英文参考文献.docVIP

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No Gold Standard Estimation of the Sensitivity and Specificity of Two Molecular Diagnostic Protocols for Trypanosoma brucei spp. in Western Kenya 英文参考文献.doc

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No Gold Standard Estimation of the Sensitivity and Specificity of Two Molecular Diagnostic Protocols for Trypanosoma brucei spp. in Western Kenya 英文参考文献

NoGoldStandardEstimationoftheSensitivityand SpecificityofTwoMolecularDiagnosticProtocolsfor Trypanosomabruceispp.inWesternKenya BarendMarkdeClareBronsvoort3*,BeatrixvonWissmann1,EricMauriceFe`vre2,IanGrahamHandel3, KimPicozzi1,SueChristinaWelburn1 1Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom, 2Centre for Infectious Diseases and CentreforInfection,ImmunityandEvolution,SchoolofBiologicalSciences,UniversityofEdinburgh,Edinburgh,UnitedKingdom,3TheRoslinInstituteandTheRoyal (Dick)SchoolofVeterinaryStudies,UniversityofEdinburgh,Roslin,Midlothian,UnitedKingdom Abstract AfricananimaltrypanosomiasisiscausedbyarangeoftsetsetransmittedprotozoanparasitesincludingTrypanosomavivax, TrypanosomacongolenseandTrypansomabrucei.InWesternKenyaandotherpartsofEastAfricatwosubspeciesofT.brucei, T.b.bruceiandthezoonoticT.b.rhodesiense,co-circulateinlivestock.Arangeofpolymerasechainreactions(PCR)havebeen developedasimportantmoleculardiagnostictoolsforepidemiologicalinvestigationsofT.heanimalreservoir andofitszoonoticpotential.QuantificationoftherelativeperformanceofdifferentdiagnosticPCRsisessentialtoensure comparabilityofstudies.ThispaperdescribesanevaluationoftwodiagnostictestsystemsforT.bruceiusingaT.bruceis.l. specificPCR[1]andasinglenestedPCRtargetingtheInternalTranscribedSpacer(ITS)regionsoftrypanosomeribosomal DNA[2].ABayesianformulationoftheHui-Walterlatentclassmodelwasemployedtoestimatetheirtestperformancein theabsenceofagoldstandardtestfordetectingT.bruceis.l.infectionsinear-veinbloodsamplesfromcattle,pig,sheepand goatpopulationsinWesternKenya,storedonWhatmanFTAcards.TheresultsindicatethatthesystememployingtheT. brucei s.l. specific PCR (Se1=0.760) had a higher sensitivity than the ITS-PCR (Se2=0.640); both have high specificity (Sp1=0.998; Sp2=0.997). The true prevalences for livestock populations were estimated (pcattle=0.091, ppigs =0.066, pgoats=0.005,psheep=0.006),takingintoaccounttheuncertainti