Non-Invasive Molecular Imaging of Fibrosis Using a Collagen-Targeted Peptidomimetic of the Platelet Collagen Receptor Glycoprotein VI 英文参考文献.docVIP

下载本文档

3
0
约4.44万字
约 10页
2017-05-12 发布于上海
举报
版权申诉

Non-Invasive Molecular Imaging of Fibrosis Using a Collagen-Targeted Peptidomimetic of the Platelet Collagen Receptor Glycoprotein VI 英文参考文献.doc

1、本文档共10页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Non-Invasive Molecular Imaging of Fibrosis Using a Collagen-Targeted Peptidomimetic of the Platelet Collagen Receptor Glycoprotein VI 英文参考文献

Non-InvasiveMolecularImagingofFibrosisUsinga Collagen-TargetedPeptidomimeticofthePlatelet CollagenReceptorGlycoproteinVI JulienMuzard1,5.,LaureSarda-Mantel2,5,6.,Ste′phaneLoyau1,5,AlainMeulemans2,5,6 ,Liliane Louedec1,5,ClaudieBantsimba-Malanda4,5,FlorenceHervatin5,Joe¨lleMarchal-Somme4,5,JeanBaptiste Michel1,5,6,DominiqueLeGuludec2,5,6,PhilippeBilliald3,MartineJandrot-Perrus1,5,6* 1INSERM,U698,Ho?pitalBichat,Paris,France,2INSERM,U773,CRB3,Faculte′ XavierBichat,Paris,France,3MuseumNationald’HistoireNaturelle,CNRS-FRE3206,Paris, France,4INSERM,U700,Faculte′ XavierBichat,Paris,France,5Universite′ Paris7,Paris,France,6AP-HP,Ho?pitalBichat,Paris,France Abstract Background:Fibrosis,whichischaracterizedbythepathologicalaccumulationofcollagen,isrecognizedasanimportant feature of many chronic diseases, and as such, constitutes an enormous health burden. We need non-invasive specific methodsfortheearlydiagnosisandfollow-upoffibrosisinvariousdisorders.Collagentargetingmoleculesarethereforeof interestforpotentialinvivoimagingoffibrosis.Inthisstudy,wedevelopedacollagen-specificprobeusinganewapproach thattakesadvantageoftheinherentspecificityofGlycoproteinVI(GPVI),themainplateletreceptorforcollagensIandIII. Methodology/PrincipalFindings:Ananti-GPVIantibodythatneutralizescollagen-bindingwasusedtoscreenabacterial random peptide library. A cyclic motif was identified, and the corresponding peptide (designated collagelin) was synthesized.Solid-phasebindingassaysandhistochemicalanalysisshowedthatcollagelinspecificallyboundtocollagen (Kd1027M)invitro,andlabelledcollagenfibersexvivoonsectionsofrataortaandrattail.Collagelinisthereforeanew specificprobeforcollagen.Thesuitabilityofcollagelinasaninvivoprobewastestedinaratmodelofhealedmyocardial infarctions(MI).InjectingTc-99m-labelledcollagelinandscintigraphicimagingshowedthatuptakeoftheprobeoccurredin thecardiacareaofratswithMI,butnotincontrols.Postmortemautoradiographyandhistologicalanalysisofheartsections showed that the labe