Non-Invasive In Vivo Imaging of Calcium Signaling in Mice 英文参考文献.docVIP

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Non-Invasive In Vivo Imaging of Calcium Signaling in Mice 英文参考文献.doc

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Non-Invasive In Vivo Imaging of Calcium Signaling in Mice 英文参考文献

Non-InvasiveInVivoImagingofCalciumSignalingin Mice KellyL.Rogers1,2¤,SandrinePicaud1,EmilieRoncali2,Raphae¨lBoisgard2,CesareColasante1,JacquesStinnakre1,BertrandTavitian2, PhilippeBru?let1* 1Unite′d’EmbryologieMole′culaire,CNRSURA2578,InstitutPasteur,Paris,France,2CEA,ServiceHospitalierFre′de′ricJoliot,Inserm,U803,Imageriede l’expressiondesge`nes,Orsay,France Rapid and transient elevations of Ca2+ within cellular microdomains play a critical role in the regulation of many signal 2+ 2+ transductionpathways.Describedhereisageneticapproachfornon-invasivedetectionoflocalizedCa concentration([Ca ]) 2+ rises in live animals using bioluminescence imaging (BLI). Transgenic mice conditionally expressing the Ca -sensitive bioluminescentreporterGFP-aequorintargetedtothemitochondrialmatrixwerestudiedinseveralexperimentalparadigms. 2+ Rapid[Ca ]risesinsidethemitochondrialmatrixcouldbereadilydetectedduringsingle-twitchmusclecontractions.Whole 2+ body patterns of [Ca ] were monitored in freely moving mice and during epileptic seizures. Furthermore, variations in 2+ mitochondrial [Ca ] correlated to behavioral components of the sleep/wake cycle were observed during prolonged whole 2+ bodyrecordingsofnewbornmice.Thisnon-invasiveimagingtechniqueopensnewavenuesfortheanalysisofCa signaling whenever whole body information in freely moving animals is desired, in particular during behavioral and developmental studies. Citation: Rogers KL, Picaud S, Roncali E, Boisgard R, Colasante C, et al (2007) Non-Invasive In Vivo Imaging of Calcium Signaling in Mice. PLoS ONE2(10):e974.doi:10.1371/journal.pone.0000974 INTRODUCTION 2+ emerged as a sensitive method for localizing gene expression or cell migration in live animals [10–12]. GFP-aequorin (GA) is Calcium(Ca )isauniversalsecondmessengerthatregulatescell signaling pathways involved in muscular contraction, hormone 2+ a bioluminescent Ca -reporter, which is based on the light 2+ emitting system of the jellyfish, Aequorea victoria [13