Notch Activation by Phenethyl Isothiocyanate Attenuates Its Inhibitory Effect on Prostate Cancer Cell Migration 英文参考文献.docVIP
- 1、本文档共12页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Notch Activation by Phenethyl Isothiocyanate Attenuates Its Inhibitory Effect on Prostate Cancer Cell Migration 英文参考文献
NotchActivationbyPhenethylIsothiocyanate
AttenuatesItsInhibitoryEffectonProstateCancerCell
Migration
Su-HyeongKim,AnuradhaSehrawat,KozueSakao,Eun-RyeongHahm,ShivendraV.Singh*
DepartmentofPharmacologyandChemicalBiology,andUniversityofPittsburghCancerInstitute,UniversityofPittsburghSchoolofMedicine,Pittsburgh,Pennsylvania,
UnitedStatesofAmerica
Abstract
Phenethylisothiocyanate(PEITC)isapromisingcancerchemopreventivecomponentofediblecruciferousvegetableswith
invivoefficacyagainstprostatecancerinexperimentalrodents.CancerchemopreventiveresponsetoPEITCischaracterized
byitsabilitytoinhibitmultipleoncogenicsignalingpathways,includingnuclearfactor-kB,Akt,andandrogenreceptor.The
presentstudydemonstrates,forthefirsttime,thatPEITCtreatmentactivatesNotchsignalinginmalignantaswellasnormal
humanprostate cells.Exposure ofhumanprostate cancer cells(LNCaP,PC-3, andDU145)andanormalhumanprostate
epithelialcellline(PrEC)toPEITCresulted incleavage(activeform) ofNotch1andNotch2,andincreasedtranscriptional
activityofNotch.InPC-3andLNCaPcells,PEITCtreatmentcausedinductionofNotchligandsJagged1andJagged2(PC-3),
overexpressionofc-secretasecomplexcomponentsPresenilin1andNicastrin(PC-3),nuclearenrichmentofcleavedNotch2,
and/orup-regulationofNotch1,Notch2,Jagged1,and/orJagged2mRNA.PEITC-inducedapoptosisinLNCaPandPC-3cells
wassignificantlyattenuatedbyRNAinterferenceofNotch2,butnotbypharmacologicalinhibitionofNotch1.Inhibitionof
PC-3andLNCaPcellmigrationresultingfromPEITCexposurewassignificantlyaugmentedbyknockdownofNotch2protein
aswellaspharmacologicalinhibitionofNotch1activation.NuclearexpressionofcleavedNotch2proteinwassignificantly
higherinPC-3xenograftsfromPEITC-treatedmiceanddorsolateralprostatesfromPEITC-fedTRAMPmicecomparedwith
respectivecontrol.BecauseNotchsignalingisimplicatedinepithelial-mesenchymaltransitionandmetastasis,thepresent
study suggests that anti-metastatic effect of PEITC may be augmented by a combination regimen involving a Notch
inhibitor.
Citation:KimS-H,SehrawatA,
您可能关注的文档
- Non-Metabolic Membrane Tubulation and Permeability Induced by Bioactive Peptides 英文参考文献.doc
- Non-Native R1 Substitution in the S4 Domain Uniquely Alters Kv4.3 Channel Gating 英文参考文献.doc
- Non-Invasive Molecular Imaging of Fibrosis Using a Collagen-Targeted Peptidomimetic of the Platelet Collagen Receptor Glycoprotein VI 英文参考文献.doc
- Non-Random Chromosome Segregation in Stem Cells 英文参考文献.doc
- Non-Negative Matrix Factorization for Learning Alignment-Specific Models of Protein Evolution 英文参考文献.doc
- Non-Redundant Role for IL-12 and IL-27 in Modulating Th2 Polarization of Carcinoembryonic Antigen Specific CD4 T Cells from Pancreatic Cancer Patients 英文参考文献.doc
- Non-Natural and Photo-Reactive Amino Acids as Biochemical Probes of Immune Function 英文参考文献.doc
- Non-Listening and Self Centered Leadership – Relationships to Socioeconomic Conditions and Employee Mental Health 英文参考文献.doc
- Non-Invasive In Vivo Imaging of Calcium Signaling in Mice 英文参考文献.doc
- Non-Parametric Change-Point Method for Differential Gene Expression Detection 英文参考文献.doc
- 新高考物理二轮复习讲练测专题12 恒定电流与交变电流(讲义)(解析版).doc
- 新高考物理二轮复习讲练测专题14 电学中三大观点的综合应用(练习)(解析版).doc
- 新高考物理二轮复习讲练测专题10 磁场 带电粒子在磁场中的运动(练习)(解析版).doc
- 新高考物理二轮复习讲练测专题21 热学和光学实验(练习)(原卷版).doc
- 新高考物理二轮复习讲练测专题17 热学(练习)(原卷版).doc
- 新高考物理二轮复习讲练测专题12 恒定电流与交变电流(练习)(解析版).doc
- 新高考物理二轮复习讲练测专题18 近代物理(练习)(解析版).doc
- 新高考物理二轮复习讲练测专题20 电学实验(练习)(解析版).doc
- 新高考物理二轮复习讲练测专题13 电磁感应(练习)(解析版).doc
- 新高考物理二轮复习讲练测专题10 磁场 带电粒子在磁场中的运动(练习)(原卷版).doc
文档评论(0)