Novel Cell-Free Strategy for Therapeutic Angiogenesis In Vitro Generated Conditioned Medium Can Replace Progenitor Cell Transplantation 英文参考文献.docVIP

下载本文档

3
0
约4.42万字
约 11页
2017-05-12 发布于上海
举报
版权申诉

Novel Cell-Free Strategy for Therapeutic Angiogenesis In Vitro Generated Conditioned Medium Can Replace Progenitor Cell Transplantation 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Novel Cell-Free Strategy for Therapeutic Angiogenesis In Vitro Generated Conditioned Medium Can Replace Progenitor Cell Transplantation 英文参考文献

NovelCell-FreeStrategyforTherapeuticAngiogenesis:In VitroGeneratedConditionedMediumCanReplace ProgenitorCellTransplantation StefanoDiSanto.,ZijiangYang.,MoritzWylervonBallmoos,JanVoelzmann,NicolasDiehm,Iris Baumgartner,ChristophKalka* DepartmentofVascularMedicine,SwissCardiovascularCenter,Inselspital,BernUniversityHospital,UniversityofBern,Bern,Switzerland Abstract Background:Currentevidencesuggeststhatendothelialprogenitorcells(EPC)contributetoischemictissuerepairbyboth secretion of paracrine factors and incorporation into developing vessels. We tested the hypothesis that cell-free administrationofparacrinefactorssecretedbyculturedEPCmayachieveanangiogeniceffectequivalenttocelltherapy. Methodology/Principal Findings: EPC-derived conditioned medium (EPC-CM) was obtained from culture expanded EPC subjected to 72hours of hypoxia. In vitro, EPC-CM significantly inhibited apoptosis of mature endothelial cells and promotedangiogenesisinarataorticringassay.ThetherapeuticpotentialofEPC-CMascomparedtoEPCtransplantation was evaluated in a rat model of chronic hindlimb ischemia. Serial intramuscular injections of EPC-CM and EPC both significantlyincreasedhindlimbbloodflowassessedbylaserDoppler(81.262.9%and83.763.0%vs.53.562.4%ofnormal, P,0.01) andimproved muscle performance. Asignificantly increased capillary density (1.6260.03 and1.6860.05/muscle fiber, P,0.05), enhanced vascular maturation (8.660.3 and 8.160.4/HPF, P,0.05) and muscle viability corroborated the findings of improved hindlimb perfusion and muscle function. Furthermore, EPC-CM transplantation stimulated the + mobilizationofbonemarrow(BM)-derivedEPCcomparedtocontrol(678.7644.1vs.340.0629.1CD34 /CD452cells/16105 + mononuclearcells,P,0.05)andtheirrecruitmenttotheischemicmuscles(5.960.7vs.2.660.4CD34 cells/HPF,P,0.001)3 daysafterthelastinjection. Conclusions/Significance: Intramuscular injection of EPC-CM is as effective as cell transplantation for promoting tissue revascularization and functional reco