Novel Cell and Tissue Acquisition System (CTAS) Microdissection of Live and Frozen Brain Tissues 英文参考文献.docVIP

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Novel Cell and Tissue Acquisition System (CTAS) Microdissection of Live and Frozen Brain Tissues 英文参考文献.doc

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Novel Cell and Tissue Acquisition System (CTAS) Microdissection of Live and Frozen Brain Tissues 英文参考文献

NovelCellandTissueAcquisitionSystem(CTAS): MicrodissectionofLiveandFrozenBrainTissues LiliC.Kudo1*,NancyVi1,ZhongcaiMa1,2,TonyFields3,NuralyK.Avliyakulov4,MichaelJ.Haykinson4, AnatolBragin1,3,StanislavL.Karsten1* 1NeuroInDx, Inc.,Signal Hill,California, UnitedStates ofAmerica, 2Division ofNeuroscience, Department ofNeurology,LosAngelesBiomedicalResearchInstituteat Harbor-UniversityofCaliforniaLosAngeles(UCLA)MedicalCenter,Torrance,California,UnitedStatesofAmerica, 3DepartmentofNeurology,DavidGeffenSchoolof Medicine at the University of California Los Angeles, Los Angeles, California, United States of America, 4Department of Biological Chemistry, David Geffen School of MedicineattheUniversityofCaliforniaLosAngeles,LosAngeles,California,UnitedStatesofAmerica Abstract We developed a novel, highly accurate, capillary based vacuum-assisted microdissection device CTAS - Cell and Tissue Acquisition System, for efficient isolation of enriched cell populations from live and freshly frozen tissues, which can be successfullyusedinavarietyofmolecularstudies,includinggenomicsandproteomics.Specificdiameterofthedisposable capillaryunit(DCU)andpreciselyregulatedshortvacuumimpulseensurecollectionofthedesiredtissueregionsandeven individualcells.WedemonstratedthatCTASiscapableofdissectingspecificregionsofliveandfrozenmouseandratbrain tissues at the cellular resolution with high accuracy. CTAS based microdissection avoids potentially harmful physical treatmentoftissuessuchaschemicaltreatment,laserirradiation,excessiveheatormechanicalcelldamage,thuspreserving primaryfunctionsandactivitiesofthedissectedcellsandtissues.HighqualityDNA,RNA,andproteincanbeisolatedfrom CTAS-dissected samples, which are suitable for sequencing, microarray, 2D gel-based proteomic analyses, and Western blotting.WealsodemonstratedthatCTAScanbeusedtoisolatecellsfromnativelivingtissuesforsubsequentrecultivation of primary cultures without affecting cellular viability, making it a simple and cost-effective