Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division 英文参考文献.docVIP

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Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division 英文参考文献.doc

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Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division 英文参考文献

NovelRoleofPhosphorylation-DependentInteraction betweenFtsZandFipAinMycobacterialCellDivision KamakshiSureka,TofajjenHossain,ParthaMukherjee,ParamitaChatterjee,PratikDatta¤ ,Manikuntala Kundu,JoyotiBasu* DepartmentofChemistry,BoseInstitute,Kolkata,India Abstract Thebacterialdivisomeisamultiproteincomplex.Specificprotein-proteininteractionsspecifywhethercelldivisionoccurs optimally,orwhetherdivisionisarrested.Littleisknownabouttheseprotein-proteininteractionsandtheirregulationin mycobacteria. We have investigated the interrelationship between the products of the Mycobacterium tuberculosis gene clusterRv0014c-Rv0019c,namelyPknA(encodedbyRv0014c)andFtsZ-interactingproteinA,FipA(encodedbyRv0019c )and theproductsofthedivisioncellwall(dcw)cluster,namelyFtsZandFtsQ.M.smegmatisstrainsdepletedincomponentsof thetwogeneclustershavebeencomplementedwithorthologsoftherespectivegenesofM.tuberculosis.Hereweidentify FipAasaninteractingpartnerofFtsZandFtsQandestablishthatPknA-dependentphosphorylationofFipAonT77andFtsZ on T343 is required for cell division under oxidative stress. A fipA knockout strain of M. smegmatis is less capable of withstanding oxidative stress than the wild type and showed elongation of cells due to a defect in septum formation. LocalizationofFtsQ,FtsZandFipAatmid-cellwasalsocompromised.Growthandsurvivaldefectsunderoxidativestress couldbefunctionallycomplementedbyfipAofM.tuberculosisbutnotitsT77Amutant.MerodiploidstrainsofM.smegmatis expressing the FtsZ(T343A) showed inhibition of FtsZ-FipA interaction and Z ring formation under oxidative stress. KnockdownofFipAledtoelongationofM.tuberculosiscellsgrowninmacrophagesandreducedintramacrophagegrowth. Thesedatarevealanovelroleofphosphorylation-dependentprotein-proteininteractionsinvolvingFipA,inthesustenance ofmycobacterialcelldivisionunderoxidativestress. Citation:SurekaK,HossainT,MukherjeeP,ChatterjeeP,DattaP,etal.(2010)NovelRoleofPhosphorylation-DependentInteractionbetweenFtsZandFipAin MycobacterialCellDivision