Novel Aptamer-Nanoparticle Bioconjugates Enhances Delivery of Anticancer Drug to MUC1-Positive Cancer Cells In Vitro 英文参考文献.docVIP

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Novel Aptamer-Nanoparticle Bioconjugates Enhances Delivery of Anticancer Drug to MUC1-Positive Cancer Cells In Vitro 英文参考文献.doc

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Novel Aptamer-Nanoparticle Bioconjugates Enhances Delivery of Anticancer Drug to MUC1-Positive Cancer Cells In Vitro 英文参考文献

NovelAptamer-NanoparticleBioconjugatesEnhances DeliveryofAnticancerDrugtoMUC1-PositiveCancer CellsInVitro ChenchenYu1.,YanHu1.,JinhongDuan1,WeiYuan2,ChenWang3,HaiyanXu1,Xian-DaYang1,3* 1InstituteofBasicMedicalSciences,ChineseAcademyofMedicalSciencesPekingUnionMedicalCollege,Beijing,China,2StateKeyLaboratoryofMolecularOncology, Cancer Institute Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China, 3National Center of Nanoscience and Technology, Beijing,China Abstract MUC1proteinisanattractivetargetforanticancerdrugdeliveryowingtoitsoverexpressioninmostadenocarcinomas.In this study, a reported MUC1 protein aptamer is exploited as the targeting agent of a nanoparticle-based drug delivery system. Paclitaxel (PTX) loaded poly (lactic-co-glycolic-acid) (PLGA) nanoparticles were formulated by an emulsion/ evaporationmethod,andMUC1aptamers(Apt)wereconjugatedtotheparticlesurfacethroughaDNAspacer.Theaptamer conjugatednanoparticles(Apt-NPs)areabout225.3nminsizewithastableinvitrodrugreleaseprofile.UsingMCF-7breast cancercellasaMUC1-overexpressingmodel,theMUC1aptamerincreasedtheuptakeofnanoparticlesintothetargetcells as measured by flow cytometry. Moreover, the PTX loaded Apt-NPs enhanced in vitro drug delivery and cytotoxicity to + MUC1 cancercells,ascomparedwithnon-targetednanoparticlesthatlacktheMUC1aptamer(P,0.01).Thebehaviorof thisnovelaptamer-nanoparticlebioconjugatessuggeststhatMUC1aptamersmayhaveapplicationpotentialintargeted drugdeliverytowardsMUC1-overexpressingtumors. Citation:YuC,HuY,DuanJ,YuanW,WangC,etal.(2011)NovelAptamer-NanoparticleBioconjugatesEnhancesDeliveryofAnticancerDrugtoMUC1-Positive CancerCellsInVitro.PLoSONE6(9):e24077.doi:10.1371/journal.pone.0024077 Editor:TarlWayneProw,UniversityofQueensland,Australia ReceivedMay2,2011;AcceptedJuly29,2011;PublishedSeptember1,2011 Copyright:?2011Yuetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distrib