Novel Rodent Models for Macular Research 英文参考文献.docVIP

NovelRodentModelsforMacularResearch GesineHuber1,SeverinHeynen2,ConiImsand2,FranziskavomHagen3,RegineMuehlfriedel1 ,Naoyuki Tanimoto1,YuxiFeng3,Hans-PeterHammes3,ChristianGrimm2,LeoPeichl4,MathiasW.Seeliger1, SusanneC.Beck1* 1DivisionofOcular Neurodegeneration,Centrefor Ophthalmology, Institute forOphthalmicResearch, University ofTuebingen, Tuebingen, Germany,2Laboratory of Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Zurich, Switzerland, 35th Medical Department, Universitatsmedizin Mannheim, University of ¨ Heidelberg,Mannheim,Germany,4MaxPlanckInstituteforBrainResearch,FrankfurtamMain,Germany Abstract Background: Many disabling human retinal disorders involve the central retina, particularly the macula. However, the commonly used rodent models in research, mouse and rat, do not possess a macula. The purpose of this study was to identifysmalllaboratoryrodentswithasignificantcentralregionaspotentialnewmodelsformacularresearch. Methodology/PrincipalFindings:Gerbillusperpallidus,MerionesunguiculatusandPhodopuscampbelli,laboratoryrodents lesscommonlyusedinretinalresearch,weresubjectedtoconfocalscanninglaserophthalmoscopy(cSLO),fluoresceinand indocyaninegreenangiography,andspectral-domainopticalcoherencetomography(SD-OCT)usingstandardequipment (HeidelbergEngineeringHRA1andSpectralisTM)adaptedtosmallrodenteyes.Theexistenceofavisualstreak-likepattern wasassessedonthebasisofvasculartopography,retinalthickness,andthetopographyofretinalganglioncellsandcone photoreceptors. All three species examined showed evidence of a significant horizontal streak-like specialization. cSLO angiography and retinal wholemounts revealed that superficial retinal blood vessels typically ramify and narrow into a sparsecapillarynetattheborderoftherespectivearealocateddorsaltotheopticnerve.Similartothemacularregion, therewasanabsenceoflargerbloodvesselsinthestreakregion.Furthermore,thethicknessofthephotoreceptorlayerand thepopulationdensityofneuronsintheganglioncelll