Nuclear Morphometric Analysis (NMA) Screening of Senescence, Apoptosis and Nuclear Irregularities 英文参考文献.docVIP
- 1、本文档共10页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Nuclear Morphometric Analysis (NMA) Screening of Senescence, Apoptosis and Nuclear Irregularities 英文参考文献
NuclearMorphometricAnalysis(NMA):Screeningof
Senescence,ApoptosisandNuclearIrregularities
EduardoC.Filippi-Chiela1,ManuelM.Oliveira3,BrunoJurkovski3,SidiaMariaCallegari-Jacques4,
ViniciusDuvaldaSilva5,GuidoLenz1,2*
1DepartmentofBiophysics,FederalUniversityofRioGrandedoSul(UFRGS),PortoAlegre,RioGrandedoSul,Brazil,2CenterofBiotechnology,FederalUniversityofRio
GrandedoSul(UFRGS),PortoAlegre,RioGrandedoSul,Brazil,3InstituteofInformatics,FederalUniversityofRioGrandedoSul(UFRGS),PortoAlegre,RioGrandedoSul,
Brazil,4GraduatePrograminGeneticsandMolecularBiology,DepartmentofStatistics,FederalUniversityofRioGrandedoSul(UFRGS),PortoAlegre,RioGrandedoSul,
Brazil,5SaoLucasHospital–Pontif?′ciaUniversidadeCato′licadoRioGrandedoSul(PUCRS),PortoAlegre,RioGrandedoSul,Brazil
Abstract
Severalcellularmechanismsaffectnuclearmorphologywhichcanthereforebeusedtoassesscertainprocesses.Here,we
presentananalytictooltoquantifythenumberofcellsinapopulationthatpresentcharacteristicsofsenescence,apoptosis
ornuclearirregularitiesthroughnuclearmorphometricanalysis.Thetoolpresentedhereisbasedonnuclearimageanalysis
andevaluationofsizeandregularityofadheredcellsinculture.From46measurementsofnuclearmorphometry,principal
component analysis filtered four measurements that best separated regular from irregular nuclei. These measurements,
namelyaspect,areabox,radiusratioandroundnesswerecombinedintoasinglenuclearirregularityindex(NII).Normal
nucleiareusedtosettheparametersforagivencelltype,anddifferentnuclearphenotypesareseparatedinanareaversus
NIIplot.Thetoolwasvalidatedwithb-galstainingforsenescenceandannexinorcaspasesinhibitorforapoptosisaswellas
severaltreatmentsthatinducedifferentcellularphenotypes.Thismethodprovidesadirectandobjectivewayofscreening
normal,senescent,apoptoticandnuclearirregularitieswhichmayoccurduringfailedmitosisormitoticcatastrophe,which
maybeveryusefulinbasicandclinicalresearch.
Citation:Filippi-ChielaEC,OliveiraMM,JurkovskiB,Callegari-JacquesSM,SilvaVDd,etal.(2012)NuclearMorphometr
您可能关注的文档
- Novel, Meso-Substituted Cationic Porphyrin Molecule for Photo-Mediated Larval Control of the Dengue Vector Aedes aegypti 英文参考文献.doc
- Novel Wireless Sensor System for Monitoring Oxygen, Temperature and Respiration Rate of Horticultural Crops Post Harvest 英文参考文献.doc
- NPR1 Protein Regulates Pathogenic and Symbiotic Interactions between Rhizobium and Legumes and Non-Legumes 英文参考文献.doc
- Now You Don't See It, Now You Do Filling In Creates the Illusion of Motion 英文参考文献.doc
- NR-2L A Two-Level Predictor for Identifying Nuclear Receptor Subfamilies Based on Sequence-Derived Features 英文参考文献.doc
- NSAID Use Selectively Increases the Risk of Non-Fatal Myocardial Infarction A Systematic Review of Randomised Trials and Observational Studies 英文参考文献.doc
- NR2C in the Thalamic Reticular Nucleus; Effects of the NR2C Knockout 英文参考文献.doc
- NSAIDs and Acute Pancreatitis A Systematic Review 英文参考文献.doc
- Nrf2 Expression Is Regulated by Epigenetic Mechanisms in Prostate Cancer of TRAMP Mice 英文参考文献.doc
- NOX2β A Novel Splice Variant of NOX2 That Regulates NADPH Oxidase Activity in Macrophages 英文参考文献.doc
- 2024年05月广东茂名信宜市广仁医院招考聘用工作人员笔试历年典型考题及考点研判与答案详解.docx
- 2024年05月广西河池市巴马县百林乡公开招聘乡级防贫监测信息员1人笔试历年典型考题及考点研判与答案详解.docx
- 2024广西博物馆实习生招募笔试备考试题及答案解析.docx
- 2024吉林通化市辉南县事业单位选调26人笔试模拟试题及答案解析.docx
- 2024贵州长顺县选聘城市社区工作者42人笔试模拟试题及答案解析.docx
- 2024天津和平区中学招聘27人笔试模拟试题及答案解析.docx
- 2024广西崇左市邮政管理局招聘1人笔试备考试题及答案解析.docx
- 2024年贵州毕节市百里杜鹃管理区事业单位招考聘用65人笔试上岸历年高频考点(难、易错点)附带答案详解.docx
- 2024年05月新疆昌吉市事业单位2024年引进急需紧缺专业人才笔试历年典型考题及考点研判与答案详解.docx
- 2024陕西师范大学吴堡实验学校教师招聘笔试备考试题及答案解析.docx
文档评论(0)