Nuclear mRNA Degradation Pathway(s) Are Implicated in Xist Regulation and X Chromosome Inactivation 英文参考文献.docVIP
- 1、本文档共9页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Nuclear mRNA Degradation Pathway(s) Are Implicated in Xist Regulation and X Chromosome Inactivation 英文参考文献
NuclearmRNADegradationPathway(s)
AreImplicatedinXistRegulation
andXChromosomeInactivation
Constance Ciaudo1,Agne`sBourdet1,Michel Cohen-Tannoudji2,Harry C.Dietz3,Claire Rougeulle1,Philip Avner1*
1Unite′deGe′ne′tiqueMole′culaireMurine,InstitutPasteur,Paris,France,2Unite′deBiologieduDe′veloppement,InstitutPasteur,Paris,France,3InstituteofGeneticMedicine
andHowardHughesMedicalInstitute,JohnsHopkinsUniversitySchoolofMedicine,Baltimore,Maryland,UnitedStatesofAmerica
A critical step in X-chromosome inactivation (XCI), which results in the dosage compensation of X-linked gene
expressioninmammals,isthecoatingofthepresumptiveinactiveXchromosomebythelargenoncodingXistRNA,
whichthenleadstotherecruitmentofotherfactorsessentialfortheheterochromatinisationoftheinactiveXandits
transcriptional silencing. In an approach aimed at identifying genes implicated in the X-inactivation process by
comparative transcriptional profiling of female and male mouse gastrula, we identified the Eif1 gene involved in
translation initiation and RNA degradation. We show here that female embryonic stem cell lines, silenced by RNA
interference for the Eif1 gene, are unable to form Xist RNA domains upon differentiation and fail to undergo X-
inactivation. To probe further an effect involving RNA degradation pathways, the inhibition by RNA interference of
Rent1,afactoressentialfornonsense-mediateddecayandExosc10,aspecificnuclearcomponentoftheexosome,was
analysedandshowntosimilarlyimpairXistupregulationandXCI.InEif1-,Rent1-,andExosc10-interferedclones,Xist
splicedform(s)arestronglydownregulated,whilethelevelsofunsplicedform(s)ofXistandthestabilityofXistRNA
remaincomparabletothatofthecontrolcelllines.OurdatasuggestsaroleformRNAnucleardegradationpathwaysin
thecriticalregulationofsplicedXistmRNAlevelsandtheonsetoftheX-inactivationprocess.
Citation: Ciaudo C, Bourdet A, Cohen-Tannoudji M, Dietz HC, Rougeulle C, et al. (2006) Nuclear mRNA degradation pathway(s) are implicated in Xist regulation and X
chrom
您可能关注的文档
- Novel, Meso-Substituted Cationic Porphyrin Molecule for Photo-Mediated Larval Control of the Dengue Vector Aedes aegypti 英文参考文献.doc
- Novel Wireless Sensor System for Monitoring Oxygen, Temperature and Respiration Rate of Horticultural Crops Post Harvest 英文参考文献.doc
- NPR1 Protein Regulates Pathogenic and Symbiotic Interactions between Rhizobium and Legumes and Non-Legumes 英文参考文献.doc
- Now You Don't See It, Now You Do Filling In Creates the Illusion of Motion 英文参考文献.doc
- NR-2L A Two-Level Predictor for Identifying Nuclear Receptor Subfamilies Based on Sequence-Derived Features 英文参考文献.doc
- NSAID Use Selectively Increases the Risk of Non-Fatal Myocardial Infarction A Systematic Review of Randomised Trials and Observational Studies 英文参考文献.doc
- NR2C in the Thalamic Reticular Nucleus; Effects of the NR2C Knockout 英文参考文献.doc
- NSAIDs and Acute Pancreatitis A Systematic Review 英文参考文献.doc
- Nrf2 Expression Is Regulated by Epigenetic Mechanisms in Prostate Cancer of TRAMP Mice 英文参考文献.doc
- NOX2β A Novel Splice Variant of NOX2 That Regulates NADPH Oxidase Activity in Macrophages 英文参考文献.doc
文档评论(0)