Oestradiol-17β plasma concentrations after intramuscular injection of oestradiol benzoate or oestradiol cypionate in llamas (Lama glama) 英文参考文献.docVIP
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Oestradiol-17β plasma concentrations after intramuscular injection of oestradiol benzoate or oestradiol cypionate in llamas (Lama glama) 英文参考文献
Cavillaetal.ActaVeterinariaScandinavica2010,52:13
/content/52/1/13
RESEARCH
OpenAccess
Oestradiol-17bplasmaconcentrationsafter
intramuscularinjectionofoestradiolbenzoateor
oestradiolcypionateinllamas(Lamaglama)
MaríaVCavilla*,CarolinaPBianchi,MarceloAAba
Abstract
Background:Llamas(Lamaglama)areinducedovulatorsandtheprocessofovulationdependsondominant
follicularsize.Inaddition,acloserelationshipbetweenbehaviouralestrusandovulationisnotregisteredinllamas.
Therefore,theexogenouscontroloffolliculardevelopmentwithhormonesaimstopredicttheoptimaltimeto
mate.Oestradiol-17b(E2)anditsestersarecurrentlyusedindomesticspecies,includingcamelids,in
synchronizationtreatments.But,inllamas,thereisnoreportsregardingtheappropriatedosagestobeusedand
mostprotocolshavebeendesignedbyextrapolationfromthoserecommendedforotherruminants.Theaimof
thepresentstudywastocharacterizeplasmaE2concentrationsinintactfemalellamasfollowingasingle
intramuscular(i.m.)injectionoftwooestradiolesters:oestradiolbenzoate(EB)andoestradiolcypionate(ECP).
Methods:Twelvenonpregnantandnonlactatingsexuallymaturellamaswerei.m.injectedonday0with2.5mg
ofEB(EBgroup,n=6)orECP(ECPgroup,n=6).Bloodsampleswerecollectedimmediatelybeforeinjection,at1,
6,12,24haftertreatmentandthendailyuntilday14postinjection.Changesinhormoneconcentrationswith
timewereanalyzedineachgroupbyanalysisofvariance(ANOVA)usingarepeatedmeasures(within-SS)design.
PlasmaE2concentrationsandareaundertheconcentration-timecurve(AUC)valueswerecomparedbetween
groupsbyANOVA.InallcasesaLeast-SignificantDifferencetest(LSD)wasusedtodeterminedifferencesbetween
means.HormonalandAUCdataareexpressedasmean±S.E.M.
Results:PeakplasmaE2concentrationswereachievedearlierandwerehigherinEBgroupthaninECPgroup.
Thereafter,E2returnedtophysiologicalconcentrationsearlierinEBgroup(day5)thaninECPgroup(day9).
AlthoughplasmaE2profilesdifferedovertimeamonggroupstherewerenodifferencesbetweenthemonAUC
values.
Conclusions:Thei.m.injectionofasingledoseofbothoestradiolestersresultedinplasmaE
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