p16 as a diagnostic marker of cervical neoplasia a tissue microarray study of 796 archival specimens 英文参考文献.docVIP

Diagnostic Pathology BioMedCentral Research Open Access p16 as a diagnostic marker of cervical neoplasia: a tissue microarray study of 796 archival specimens Iana Lesnikova*1, Marianne Lidang2, Stephen Hamilton-Dutoit1 and J?rn Koch1 Address: 1Institute of Pathology, Aarhus University Hospital, Aarhus, Denmark and 2Institute of Pathology, Herlev Hospital, Herlev, Denmark Email: Iana Lesnikova* - janalni@yahoo.dk; Marianne Lidang - marlid01@heh.regionh.dk; Stephen Hamilton-Dutoit - stephami@rm.dk; J?rn Koch - joerkoch@rm.dk * Corresponding author Published: 9 July 2009 Received: 7 May 2009 Accepted: 9 July 2009 Diagnostic Pathology 2009, 4:22 doi:10.1186/1746-1596-4-22 This article is available from: /content/4/1/22 ? 2009 Lesnikova et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: To evaluate the usefulness of this biomarker in the diagnosis of cases of cervical neoplasia we studied the immunohistochemical expression of p16INK4a in a large series of archival cervical biopsies arranged into tissue microarray format. Methods: TMAs were constructed with tissue cores from archival formalin fixed, paraffin- embedded donor tissues from 796 patients, and included cases of cervical intraepithelial neoplasia (CIN)1 (n = 249), CIN2 (n = 233), CIN3 (n = 181), and invasive cervical carcinoma (n = 133). p16INK4a expression was scored using two different protocols: 1) positive vs negative p16INK4a staining; 2) a semi-quantitative immunohistochemical score (0 to 8 points) according to the intensity of staining and the proportion of stained cells Results: p16INK4A expression was not seen in normal cervix tissue, but was found with increasing frequency in t