Pathological and immunohistochemical study of lethal primary brain stem injuries 英文参考文献.docVIP

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Pathological and immunohistochemical study of lethal primary brain stem injuries 英文参考文献.doc

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Pathological and immunohistochemical study of lethal primary brain stem injuries 英文参考文献

Rongchaoetal.DiagnosticPathology2012,7:54 /content/7/1/54 RESEARCH OpenAccess Pathologicalandimmunohistochemicalstudyof lethalprimarybrainsteminjuries SunRongchao*,YangShudongandZhouZhiyi Abstract Background:Manyofthedeathsthatoccurshortlyafterinjuryorinhospitalsarecausedbymildtrauma.Slight morphologicalchangesareoftenfoundinthebrainstemsofthesepatientsduringautopsy.Thepurposeofthis studyistoinvestigatethehistopathologicalchangesinvolvedinprimarybrainsteminjuries(PBSI)andtheir diagnosticsignificance. Methods:Atotalof65patientswhohaddiedofPBSIandotherconditionswererandomlyselected.Theywere dividedinto2groups,aninjurygroup(25cases)andacontrolgroup(20cases).Slidesofeachpatient’ smidbrain, pons,andmedullaoblongatawerepreparedandstainedwithHE,argentaffin,andimmunohistochemicalagents (GFAP,NF,amyloid-?,MBP).Underlowpower(×100)andNFstaining,thediameterofthethickestlongitudinal axonwasmeasuredatitswidestpoint.Tensuchdiameterswerecollectedforeachpartofthebrain(midbrain, pons,andmedullaoblongata).Datawererecordedandanalyzedstatistically. Results:Brainstemcontusions,astrocyteactivity,edema,andpathologicalchangesintheneuronswerevisibly differentintheinjuryandcontrolgroups(P0.05).Characteristicchangesoccurredintheneuralaxons,axon diametervariedfromaxontoaxonandevenoverdifferentsegmentsofoneaxon,andseveralpathological phenomenawereobserved.Theseincludedsegmentalthickeningandcurving,wave-likeprocessing, disarrangement,andirregularswelling.Afewaxonsrupturedandintumescedintoretractionballs. ImmunohistochemicalMBPstainingshowedenlargementandcurvingofspacesbetweenthemyelinsheathsand axonsincertainareas.Themyelinsheathsliningthesurfacesoftheaxonswereinsomecasesincompleteand evenexfoliated,andsegmentationdisappeared.Thesepathologicalchangesincreasedinseverityovertime (P0.05). Conclusions:ThesehistopathologicalchangesmayprovebeneficialtothepathologicaldiagnosisofPBSIduring autopsy.Themeasurementofaxondiametersprovidesareferentquantitativeindexforthediagnosisofthespecific causesofdeathi