Patterned Cell Adhesion Associated with Tissue Deformations during Dorsal Closure in Drosophila 英文参考文献.docVIP

PatternedCellAdhesionAssociatedwithTissue DeformationsduringDorsalClosureinDrosophila AnaMargaridaMateus1,2,AlfonsoMartinezArias1* 1DepartmentofGenetics,UniversityofCambridge,Cambridge,UnitedKingdom,2GulbenkianPhDProgrammeinBiomedicine,Oeiras,Portugal Abstract Cell shape changes within epithelia require the regulation of adhesive molecules that maintain tissue integrity. How remodelling of cell contacts is achieved while tissue integrity is maintained remains a fundamental question in morphogenesis.DorsalClosureisagoodsystemtostudythedynamicsofDE-Cadherinduringmorphogenesis.Itrelieson concerted cell shape changes of two epithelial sheets: amnioserosa cell contraction and epidermal cell elongation. To investigate the modulation of DE-Cadherin we performed antibody uptake experiments in live embryos during Dorsal Closure.WefoundthatsomeantibodiesaccesscertainepitopesoftheextracellulardomainofnativeDE-Cadherinonlyin theamnioserosaandepidermalcellsattachedtotheamnioserosa,whichhasneverbeenobservedinfixedDE-Cadherinin Drosophilaembryos.Thesedifferencescorrelatewiththedifferentcellbehaviouroftheseregionsandthereforewesuggest that DE-Cadherin exists in different forms that confer different adhesive strengths. We propose this to be a widespread mechanismforthedifferentialmodulationofadhesionduringmorphogenesis. Citation:MateusAM,MartinezAriasA(2011)PatternedCellAdhesionAssociatedwithTissueDeformationsduringDorsalClosureinDrosophila.PLoSONE6(11): e27159.doi:10.1371/journal.pone.0027159 Editor:EdwardGiniger,NationalInstitutesofHealth(NIH),UnitedStatesofAmerica ReceivedApril26,2011;AcceptedOctober11,2011;PublishedNovember4,2011 Copyright: ? 2011 Mateus, Martinez Arias. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwasfundedbyFundac?a?oparaaCie?nciaeTecnologiafromPortugal(AMM)andtheWellco