Phaeophytin Analogues from Ligularia knorringiana 英文参考文献.docVIP

Molecules 2012, 17, 5219-5224; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Phaeophytin Analogues from Ligularia knorringiana Hui Li 1, Lina Li 1, Qiusheng Zheng 1, Chiaki Kuroda 2 and Qi Wang 1,* 1 Key Laboratory of Xin Jiang Phytomedicine Resources, School of Pharmacy, Shihezi University, Shihezi 832002, China; E-Mails: xiaohuiyaoshi@163.com (H.L.); 214594367@ (L.L.); johnsonjem@ (Q.Z.) 2 Department of Chemistry, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan; E-Mail: kuroda5000144@grp.rikkyo.ne.jp * Author to whom correspondence should be addressed; E-Mail: qiw16@163.com; Tel.: +86-993-205-7330. Received: 15 March 2012; in revised form: 25 April 2012 / Accepted: 27 April 2012 / Published: 7 May 2012 Abstract: A new phaeophytin, ligulariaphytin A, together with five known phaeophytins, were isolated from the aerial parts of Ligularia knorringiana. The structure of ligulariaphytin A was elucidated as 131-hydroxy-131,132-peroxyphaeophorbide A ethyl ester (1), and the five known compounds were identified as 132-hydroxyphaeophorbide A ethyl ester (2), 173-ethoxyphaeophorbide A (3), phaeophytin B (4), phaeophytin A (5), and phaeophorbide B ethyl ester (6), respectively, based on spectroscopic analysis and by comparison of their spectral data with those reported previously in the literature. All compounds were evaluated for their in vitro cytotoxic activities against cultured Hela cell, and were found to show only very weak cytotoxicity. Keywords: Ligularia knorringiana; phaeophytins; ligulariaphytin A 1. Introduction The genus Ligularia Cass. is highly diversified in China, consisting of about 110 species [1]. More than 40 species of Ligularia have been studied chemically, and more than 300 compounds, including terpenoids, pyrrolizidine alkaloids, flavonoids and benzofuran derivatives, have been isolated, with a variety of