Phagocytosis and Respiratory Burst Activity in Lumpsucker (Cyclopterus lumpus L.) Leucocytes Analysed by Flow Cytometry 英文参考文献.docVIP

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Phagocytosis and Respiratory Burst Activity in Lumpsucker (Cyclopterus lumpus L.) Leucocytes Analysed by Flow Cytometry 英文参考文献.doc

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Phagocytosis and Respiratory Burst Activity in Lumpsucker (Cyclopterus lumpus L.) Leucocytes Analysed by Flow Cytometry 英文参考文献

PhagocytosisandRespiratoryBurstActivityin Lumpsucker(CyclopteruslumpusL.)Leucocytes AnalysedbyFlowCytometry GyriT.Haugland1*,RagnhildAakreJakobsen1,NilsVestvik2,KristianUlven1,LeneStokka1, HeidrunI.Wergeland1 1DepartmentofBiology,UniversityofBergen,Bergen,Norway,2AquaKompetanseAS,Flatanger,Norway Abstract In the present study, we have isolated leucocytes from peripheral blood, head kidney and spleen from lumpsucker (CyclopteruslumpusL.),andperformedfunctionalstudieslikephagocytosisandrespiratoryburst,aswellasmorphological and cytochemical analyses. Different leucocytes were identified, such as lymphocytes, monocytes/macrophages and polymorphonuclearcells withbeanshapedorbilobed nuclei.Inaddition,cellswithsimilarmorphologyasdescribed for dendriticcellsintroutwereabundantamongtheisolatedleucocytes.Flowcytometrywassuccessfullyusedformeasuring phagocytosis and respiratory burst activity. The phagocytic capacity and ability were very high, and cells with different morphology in all three leucocyte preparations phagocytised beads rapidly. Due to lack of available cell markers, the identity of the phagocytic cells could not be determined. The potent non-specific phagocytosis was in accordance with ahighnumberofcellspositiveformyeloperoxidase,anenzymeinvolvedinoxygen-dependentkillingmechanismpresent inphagocyticcells.Further,highrespiratoryburstactivitywaspresentintheleucocytessamples,verifyingapotentoxygen- dependentdegradation.Atpresent,thespecificantibodyimmuneresponsecouldnotbemeasured,asimmunoglobulinor B-cellshavenot yetbeenisolated. Therefore, analysesof thespecific immuneresponse in thisfish species awaitfurther clarification. The present study presents the first analyses of lumpsucker immunity and also the first within the order Scopaeniformes. Citation:HauglandGT,JakobsenRA,VestvikN,UlvenK,StokkaL,etal.(2012)PhagocytosisandRespiratoryBurstActivityinLumpsucker(CyclopteruslumpusL.) LeucocytesAnalysedbyFlowCytometry.PLoSONE7(10):e47909.doi:10.1371/journal.p