Population Diversity of Rice Stripe Virus-Derived siRNAs in Three Different Hosts and RNAi-Based Antiviral Immunity in Laodelphgax striatellus 英文参考文献.docVIP
- 1、本文档共11页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Population Diversity of Rice Stripe Virus-Derived siRNAs in Three Different Hosts and RNAi-Based Antiviral Immunity in Laodelphgax striatellus 英文参考文献
PopulationDiversityofRiceStripeVirus-DerivedsiRNAs
inThreeDifferentHostsandRNAi-BasedAntiviral
ImmunityinLaodelphgaxstriatellus
YiXu1,LingzheHuang1,ShuaiFu1,JianxiangWu1*,XuepingZhou1,2*
1StateKeyLaboratoryofRiceBiology,InstituteofBiotechnology,ZhejiangUniversity,Hangzhou,China,2StateKeyLaboratoryforBiologyofPlantDiseasesandInsect
Pests,InstituteofPlantProtection,ChineseAcademyofAgriculturalSciences,Beijing,China
Abstract
Background: Small RNA-mediated gene silencing plays evolutionarily conserved roles in gene regulation and defense
againstinvasivenucleicacids.Virus-derivedsmallinterferingRNAs(vsiRNAs)areoneofthekeyelementsinvolvedinRNA
silencing-basedantiviralactivitiesinplantandinsect.vsiRNAsproducedaftervirusesinfectinghostsfromasinglekingdom
(i.e.,plantoranimal)arewelldescribed.Incontrast,vsiRNAsderivedfromvirusescapableofinfectingbothplantsandtheir
insectvectorshavenotbeencharacterized.
Methodology/Principal Findings: We examined Rice stripe virus (RSV)-derived small interfering RNAs in three different
hosts, Oryza sativa, Nicotiana benthamiana and a natural RSV transmitting vector Laodelphgax striatellus, through deep
sequencing.OurresultsshowthatlargeamountsofvsiRNAsgeneratedinthesehostsafterRSVinfection.ThevsiRNAsfrom
N. benthamiana and L. striatellus mapped equally to the genomic- and antigenomic-strand of RSV RNAs. They showed,
however, a significant bias in those from O. sativa. Furthermore, our results demonstrate that the number and size
distributionsofvsiRNAsinthethreehostswereverydifferent.InO.sativaandN.benthamiana,mostvsiRNAsweremapped
tothediscreteregionsintheRSVgenomesequence,andmostofthevsiRNAsfromthesetwohostsweregeneratedfrom
RSVgenomicRNAs3and4.Incontrast,thevsiRNAsidentifiedinL.striatellusdistributeduniformlyalongthewholegenome
ofRSV.WehavealsoshownthatsilencingAgronaute2inL.striatellusenhancedRSVaccumulationinthishost.
Conclusions/Significance:OurstudydemonstratesthatthecoreRNA-inducedgenesilencing(RNAi)machineryispresentin
L.striatellus.
您可能关注的文档
- Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso 英文参考文献.doc
- Polyphosphates as a source of enhanced P fluxes in marine sediments overlain by anoxic waters Evidence from 31P NMR 英文参考文献.doc
- Polysaccharide-Based Hydrogels The Key Role of Water in Affecting Mechanical Properties 英文参考文献.doc
- Polypyrimidine Tract Binding Protein Functions as a Negative Regulator of Feline Calicivirus Translation 英文参考文献.doc
- Polystyrene Based Silver Selective Electrodes 英文参考文献.doc
- Polysome Profiling in Liver Identifies Dynamic Regulation of Endoplasmic Reticulum Translatome by Obesity and Fasting 英文参考文献.doc
- Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells 英文参考文献.doc
- Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae 英文参考文献.doc
- Polysilane Dendrimers 英文参考文献.doc
- Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes 英文参考文献.doc
最近下载
- 《改造我们的学习》课件+2024-2025学年统编版高中语文选择性必修中册.pptx VIP
- T∕CECS 10028-2019 绿色建材评价 钢结构房屋用钢构件(可复制版).pdf
- 毕业典礼的活动策划方案.pptx VIP
- A4线缆标签打印模板.xls VIP
- 2022年高中学业水平考试试题及答案9科.docx VIP
- 临潭县住房和城乡建设局社会稳定风险评估工作实施方案.pdf VIP
- 新北区苏科版二年级上册劳动《6、树叶书签》教案.docx VIP
- 试验设计与数据处理(第三版)李云雁-第4章-回归分析.pptx VIP
- 西子奥的斯OH-CON电气原理图纸XAA21310S 一体化LMCB图纸.pdf
- A4标签打印模板.xls VIP
文档评论(0)