Porous Bead-Based Diagnostic Platforms Bridging the Gaps in Healthcare 英文参考文献.docVIP

Sensors 2012, 12, 15467-15499; doi:10.3390/s121115467 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Porous Bead-Based Diagnostic Platforms: Bridging the Gaps in Healthcare Jie Chou 1, Jorge Wong 2, Nicolaos Christodoulides 1,3, Pierre N. Floriano 1,3, Ximena Sanchez 1,3 and John McDevitt 1,3, * 1 Department of Bioengineering, Rice University, 6100 Main St MS-142, Houston, TX 77005, USA; E-Mails: jie.chou@ (J.C.); christo@ (N.C.); pfloriano@ (P.N.F.); vsan@ (X.S.) 2 3 Department of Chemistry, University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA; E-Mail: jwongc@ Department of Chemistry, Rice University, 6100 Main St MS-142, Houston, TX 77005, USA * Author to whom correspondence should be addressed; E-Mail: mcdevitt@; Tel.: +1-713-348-2123; Fax: +1-713-348-2302. Received: 1 September 2012; in revised form: 25 October 2012 / Accepted: 1 November 2012 / Published: 9 November 2012 Abstract: Advances in lab-on-a-chip systems have strong potential for multiplexed detection of a wide range of analytes with reduced sample and reagent volume; lower costs and shorter analysis times. The completion of high-fidelity multiplexed and multiclass assays remains a challenge for the medical microdevice field; as it struggles to achieve and expand upon at the point-of-care the quality of results that are achieved now routinely in remote laboratory settings. This review article serves to explore for the first time the key intersection of multiplexed bead-based detection systems with integrated microfluidic structures alongside porous capture elements together with biomarker validation studies. These strategically important elements are evaluated here in the context of platform generation as suitable for near-patient testing. Essential issues related to the scalability of these modular sensor ensembles are explored as are attempts to move such multiplexed and multiclass pl