PR3-ANCA in Wegeners granulomatosis prime human mononuclear cells for enhanced activation via TLRs and NOD12 英文参考文献.docVIP

Diagnostic Pathology BioMedCentral Research Open Access PR3-ANCA in Wegeners granulomatosis prime human mononuclear cells for enhanced activation via TLRs and NOD1/2 Akiko Uehara*, Tadasu Sato, Atsushi Iwashiro and Sou Yokota Address: Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan Email: Akiko Uehara* - kyoro@mail.tains.tohoku.ac.jp; Tadasu Sato - kyoro@mail.tains.tohoku.ac.jp; Atsushi Iwashiro - kyoro@mail.tains.tohoku.ac.jp; Sou Yokota - kyoro@mail.tains.tohoku.ac.jp * Corresponding author Published: 14 July 2009 Received: 2 June 2009 Accepted: 14 July 2009 Diagnostic Pathology 2009, 4:23 doi:10.1186/1746-1596-4-23 This article is available from: /content/4/1/23 ? 2009 Uehara et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Anti-neutrophil cytoplasmic antibodies (ANCA) is autoantibodies characteristic of vasculitis diseases. A connection between ANCA and Wegeners granulomatosis was well established. The interaction of both ANCA phenotypes (PR3-ANCA and MPO-ANCA) with leukocytes provoked cell activation, which might be involved in the pathogenesis of ANCA-related Wegeners granulomatosis. Methods: In this study, we examined whether PR3-ANCA sera and purified immunoglobulins from patients with Wegeners granulomatosis prime human monocytic cells for enhanced responses to microbial components in terms of production of proinflammatory cytokines. Results: Flow cytometry demonstrated that stimulation with antibodies to proteinase 3 enhanced the expression of TLR2, 3, 4, 7, and 9, NOD1, and NOD2 in human mononuclear cells. The sera and purified immunoglobulins significantly primed human monon