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Preferentially Quantized Linker DNA Lengths in Saccharomyces cerevisiae 英文参考文献
PreferentiallyQuantizedLinkerDNALengthsin
Saccharomycescerevisiae
Ji-PingWang1*,YvonneFondufe-Mittendorf2,3,LiqunXi1,Guei-FengTsai4,EranSegal5 ,Jonathan
Widom2,3
*
1Department of Statistics, Northwestern University, Evanston, Illinois, United States of America, 2Department of Biochemistry, Molecular Biology and Cell Biology,
NorthwesternUniversity,Evanston,Illinois,UnitedStatesofAmerica,3DepartmentofChemistry,NorthwesternUniversity,Evanston,Illinois,UnitedStatesofAmerica,
4CenterforDrugEvaluation,Taipei,Taiwan,RepublicofChina,5DepartmentofComputerScienceandAppliedMathematics,WeizmannInstituteofScience,Rehovot,
Israel
Abstract
The exact lengths of linker DNAs connecting adjacent nucleosomes specify the intrinsic three-dimensional structures of
eukaryoticchromatinfibers.SomestudiessuggestthatlinkerDNAlengthspreferentiallyoccuratcertainquantizedvalues,
differingonefromanotherbyintegralmultiplesoftheDNAhelicalrepeat,,10bp;however,studiesintheliteratureare
inconsistent.Here,weinvestigatelinkerDNAlengthdistributionsintheyeastSaccharomycescerevisiaegenome,usingtwo
novelmethods:aFourieranalysisofgenomicdinucleotideperiodicitiesadjacenttoexperimentallymappednucleosomes
andadurationhiddenMarkovmodelappliedtoexperimentallydefineddinucleosomes.Bothmethodsrevealthatlinker
DNAlengthsinyeastarepreferentiallyperiodicattheDNAhelicalrepeat(,10bp),obeyingtheforms10n+5bp(integern).
This10bpperiodicityimpliesanorderedsuperhelicalintrinsicstructurefortheaveragechromatinfiberinyeast.
Citation: WangJ-P,Fondufe-MittendorfY,XiL,TsaiG-F,SegalE,etal.(2008)PreferentiallyQuantizedLinkerDNALengthsin Saccharomycescerevisiae .PLoS
ComputBiol4(9):e1000175.doi:10.1371/journal.pcbi.1000175
Editor:GaryStormo,WashingtonUniversity,UnitedStatesofAmerica
ReceivedApril18,2008;AcceptedAugust1,2008;PublishedSeptember12,2008
Copyright: ? 2008 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricteduse,distribution
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